Activation of Human Prorenin by Lipidic Constituents of the Cell Membrane

N. Glorioso, C. Troffa, G. Tonolo, P. Manunta, P. Madeddu, M. G. Melts, A. Pazzola, F. Pala, M. Maioli, P. Mameli, S. Salardi, G. Bianchi

Research output: Contribution to journalArticle

Abstract

Activation of semipurified human kidney prorenin was found to occur in vitro in presence of a mixture of lipids that mimics the composition of t h e inner human cell membrane. The lipid-dependent activation was indeed only partial (38 ± 4%) when compared to that obtained by trypsin in liquid phase (100 µg/mL) used as a control of maximal activation (100%) under our experimental conditions (semipurified human kidney prorenin in presence of semipurified human plasma renin substrate at a concentration of 1400 ng/mL, at pH 7.2). The phenomenon was time-dependent up to 60 min whereas the angiotensin I generated after 120 min was virtually the same as that generated after 60 min thus indicating a possible reversible activation of human prorenin. We speculate that prorenin may be reversibly activated b y contact with the lipidic portion of the cell membrane either inside or outside the cells thus allowing a limited angiotensin II-generating cascade at a local site initiated by prorenin independently from the presence of active renin. Am J Hypertens 1989; 2: 920-923.

Original languageEnglish
Pages (from-to)920-923
Number of pages4
JournalAmerican Journal of Hypertension
Volume2
Issue number12
DOIs
Publication statusPublished - 1989

Keywords

  • Angiotensin II
  • Inactive rennin
  • Lipidic vesicles
  • Prorenin
  • Reversible activation

ASJC Scopus subject areas

  • Internal Medicine
  • Cardiology and Cardiovascular Medicine

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    Glorioso, N., Troffa, C., Tonolo, G., Manunta, P., Madeddu, P., Melts, M. G., Pazzola, A., Pala, F., Maioli, M., Mameli, P., Salardi, S., & Bianchi, G. (1989). Activation of Human Prorenin by Lipidic Constituents of the Cell Membrane. American Journal of Hypertension, 2(12), 920-923. https://doi.org/10.1093/ajh/2.12.920