Activation of major histocompatibility complex class I mRNA containing an Alu-like repeat in polyoma virus-transformed rat cells

B. Majello, G. la Mantia, A. Simeone, E. Boncinelli, L. Lania

Research output: Contribution to journalArticle

Abstract

Class I genes of the major histocompatibility complex (MHC) appear to be activated in mouse cells transformed by the DNA tumour virus simian virus 40 (SV40). Conversely, suppression of MHC class I genes has been reported in adenovirus-12-transformed baby kidney rat cells. We have now investigated the expression of genes encoded by the rat MHC locus in rat fibroblasts cells transformed by polyoma virus (Py). Using a mouse genomic H-2 clone as a probe in Northern transfer hybridization analysis, we have observed a high level of expression of rat MHC class I messenger RNA in all the transformed rat cell lines analysed. The class I 1.6-kilobase (kb) mRNA activated in Py-transformed rat cells appears to contain an Alu-like type II repeat element, as the same 1.6-kb mRNA is detected using either the H-2 class I sequence or a repetitive Alu-like type II element as a probe. High levels of heterogeneous poly(A)+ transcripts of 0.5-0.8 kb are also observed in Py-transformed rat cells using probes containing an Alu-like type II repetitive element.

Original languageEnglish
Pages (from-to)457-459
Number of pages3
JournalNature
Volume314
Issue number6010
DOIs
Publication statusPublished - 1985

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Polyomavirus
Major Histocompatibility Complex
Messenger RNA
MHC Class I Genes
DNA Tumor Viruses
Transformed Cell Line
Poly A
Simian virus 40
Adenoviridae
Clone Cells
Fibroblasts
Kidney
Gene Expression

ASJC Scopus subject areas

  • General

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Activation of major histocompatibility complex class I mRNA containing an Alu-like repeat in polyoma virus-transformed rat cells. / Majello, B.; la Mantia, G.; Simeone, A.; Boncinelli, E.; Lania, L.

In: Nature, Vol. 314, No. 6010, 1985, p. 457-459.

Research output: Contribution to journalArticle

Majello, B. ; la Mantia, G. ; Simeone, A. ; Boncinelli, E. ; Lania, L. / Activation of major histocompatibility complex class I mRNA containing an Alu-like repeat in polyoma virus-transformed rat cells. In: Nature. 1985 ; Vol. 314, No. 6010. pp. 457-459.
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