Activation of Metalloproteinases-2 and -9 by Interleukin-1α in S100A4-positive Liposarcoma Cell Line: Correlation with Cell Invasiveness

Laura Pazzaglia, Francesca Ponticelli, Giovanna Magagnoli, Giorgia Magagnoli, Gabriella Gamberi, Paola Ragazzini, Alba Balladelli, Piero Picci, Mariaserena Benassi

Research output: Contribution to journalArticle

Abstract

Background: The S100A4 gene may affect the invasive properties of tumor cells through modulation of metalloproteinases (MMPs) and their inhibitors (TIMPs). Materials and Methods: In the human liposarcoma cell line, SW872, we analyzed the expression of S100A4 protein by immunohistochemistry and flow cytometry. The production of MMP2, MMP9, TIMP1 and TIMP2 was assessed by gelatin zymography and enzyme-linked immunoabsorbent assay before and after interleukin-1α (IL-1α) and interleukin-6 (IL-6) stimulation; cell invasiveness was measured by Matrigel invasion assay. Results: S100A4-positive SW872 cells responded to IL-1α with induction of immunoreactive MMP2 and TIMP1 and with activation of both MMPs, the latter significantly associated with an increase of cell invasiveness. Treatment with IL-6 induced less significant variations resulting in a more stable invasive behavior. Conclusion: These data show that S100A4-positive SW872 respond to interleukins by influencing the behavior of factors involved in extracellular matrix degradation and emphasize the predominant role of MMP activity status on the positive regulation of cell migration mechanisms.

Original languageEnglish
Pages (from-to)967-972
Number of pages6
JournalAnticancer Research
Volume24
Issue number2 B
Publication statusPublished - Mar 2004

Keywords

  • Extracellular matrix
  • Liposarcoma cells
  • Metalloproteinases
  • S100A4 protein

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint Dive into the research topics of 'Activation of Metalloproteinases-2 and -9 by Interleukin-1α in S100A4-positive Liposarcoma Cell Line: Correlation with Cell Invasiveness'. Together they form a unique fingerprint.

  • Cite this