TY - JOUR
T1 - Activation of nitric oxide synthase is involved in tamoxifen-induced apoptosis of human erythroleukemia K562 cells
AU - Maccarrone, Mauro
AU - Fantini, Cristina
AU - Ranalli, Marco
AU - Melino, Gerry
AU - Agrò, Alessandro Finazzi
PY - 1998/9/4
Y1 - 1998/9/4
N2 - Tamoxifen induces apoptosis (programmed cell death) in human erythroleukemia K562 cells. Nitric oxide synthase activity and expression increased in apoptotic cells by 315% and 280%, respectively, compared to controls. The specific inhibitor of nitric oxide synthase, l-NAME, protected K562 cells from tamoxifen-induced apoptosis, whereas the nitric oxide donor, sodium nitroprusside (SNP), potentiated the apoptotic effect of the drug. In addition, 5-lipoxygenase was activated by tamoxifen and the specific enzyme inhibitor, MK886, protected K562 cells against the drug. Conversely, the 5-lipoxygenase product, 5-hydroperoxyeicosatetraenoic acid, enhanced the tamoxifen-induced apoptosis. Finally, tamoxifen altered also membrane properties of K562 cells. Copyright (C) 1998 Federation of European Biochemical Societies.
AB - Tamoxifen induces apoptosis (programmed cell death) in human erythroleukemia K562 cells. Nitric oxide synthase activity and expression increased in apoptotic cells by 315% and 280%, respectively, compared to controls. The specific inhibitor of nitric oxide synthase, l-NAME, protected K562 cells from tamoxifen-induced apoptosis, whereas the nitric oxide donor, sodium nitroprusside (SNP), potentiated the apoptotic effect of the drug. In addition, 5-lipoxygenase was activated by tamoxifen and the specific enzyme inhibitor, MK886, protected K562 cells against the drug. Conversely, the 5-lipoxygenase product, 5-hydroperoxyeicosatetraenoic acid, enhanced the tamoxifen-induced apoptosis. Finally, tamoxifen altered also membrane properties of K562 cells. Copyright (C) 1998 Federation of European Biochemical Societies.
KW - 5-Lipoxygenase
KW - Apoptosis
KW - Biomembrane
KW - Cholesterol
KW - Nitric oxide synthase
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U2 - 10.1016/S0014-5793(98)01026-6
DO - 10.1016/S0014-5793(98)01026-6
M3 - Article
C2 - 9742967
AN - SCOPUS:0032441206
VL - 434
SP - 421
EP - 424
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -