Abstract
The role of ATP on regulation of the Na+/K+-ATPase activity in the human cancerous HeLa cells was investigated. HeLa cells stimulated with increasing ATP concentrations showed a dose-dependent inhibition of the Na+/K+-ATPase activity. These effects were also obtained by UTP. ATP and UTP provoked a rise in intracellular calcium concentration ([Ca2+]i) persisting for at least 4 min. The inhibitor of phospholipase C, U73122, blocked the elevation of [Ca2+]i provoked by ATP/UTP. The expression of mRNA for P2Y2 and P2Y6 receptors was demonstrated by RT-PCR. ATP/UTP activated PKC-α, -βI and -ε isoforms, but not PKC-δ and -ζ. The inhibition of the Na+/K+-ATPase activity by ATP/UTP was blocked by Gö6976, a specific inhibitor of the calcium-dependent PKCs. In conclusion, our results suggest that ATP/UTP modulate Na+/K+-ATPase activity in HeLa cells through the P2Y2 purinoceptor via calcium mobilisation and activation of calcium-dependent PKCs.
| Original language | English |
|---|---|
| Pages (from-to) | 115-121 |
| Number of pages | 7 |
| Journal | Cellular Signalling |
| Volume | 15 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2003 |
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Keywords
- ATP
- HeLa
- Na/K ATPase
ASJC Scopus subject areas
- Cell Biology
Cite this
Activation of P2Y2 purinoceptor inhibits the activity of the Na+/K+-ATPase in HeLa cells. / Muscella, Antonella; Elia, Maria Giovanna; Greco, Simona; Storelli, Carlo; Marsigliante, Santo.
In: Cellular Signalling, Vol. 15, No. 1, 01.01.2003, p. 115-121.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Activation of P2Y2 purinoceptor inhibits the activity of the Na+/K+-ATPase in HeLa cells
AU - Muscella, Antonella
AU - Elia, Maria Giovanna
AU - Greco, Simona
AU - Storelli, Carlo
AU - Marsigliante, Santo
PY - 2003/1/1
Y1 - 2003/1/1
N2 - The role of ATP on regulation of the Na+/K+-ATPase activity in the human cancerous HeLa cells was investigated. HeLa cells stimulated with increasing ATP concentrations showed a dose-dependent inhibition of the Na+/K+-ATPase activity. These effects were also obtained by UTP. ATP and UTP provoked a rise in intracellular calcium concentration ([Ca2+]i) persisting for at least 4 min. The inhibitor of phospholipase C, U73122, blocked the elevation of [Ca2+]i provoked by ATP/UTP. The expression of mRNA for P2Y2 and P2Y6 receptors was demonstrated by RT-PCR. ATP/UTP activated PKC-α, -βI and -ε isoforms, but not PKC-δ and -ζ. The inhibition of the Na+/K+-ATPase activity by ATP/UTP was blocked by Gö6976, a specific inhibitor of the calcium-dependent PKCs. In conclusion, our results suggest that ATP/UTP modulate Na+/K+-ATPase activity in HeLa cells through the P2Y2 purinoceptor via calcium mobilisation and activation of calcium-dependent PKCs.
AB - The role of ATP on regulation of the Na+/K+-ATPase activity in the human cancerous HeLa cells was investigated. HeLa cells stimulated with increasing ATP concentrations showed a dose-dependent inhibition of the Na+/K+-ATPase activity. These effects were also obtained by UTP. ATP and UTP provoked a rise in intracellular calcium concentration ([Ca2+]i) persisting for at least 4 min. The inhibitor of phospholipase C, U73122, blocked the elevation of [Ca2+]i provoked by ATP/UTP. The expression of mRNA for P2Y2 and P2Y6 receptors was demonstrated by RT-PCR. ATP/UTP activated PKC-α, -βI and -ε isoforms, but not PKC-δ and -ζ. The inhibition of the Na+/K+-ATPase activity by ATP/UTP was blocked by Gö6976, a specific inhibitor of the calcium-dependent PKCs. In conclusion, our results suggest that ATP/UTP modulate Na+/K+-ATPase activity in HeLa cells through the P2Y2 purinoceptor via calcium mobilisation and activation of calcium-dependent PKCs.
KW - ATP
KW - HeLa
KW - Na/K ATPase
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UR - http://www.scopus.com/inward/citedby.url?scp=0037209653&partnerID=8YFLogxK
U2 - 10.1016/S0898-6568(02)00062-1
DO - 10.1016/S0898-6568(02)00062-1
M3 - Article
VL - 15
SP - 115
EP - 121
JO - Cellular Signalling
JF - Cellular Signalling
SN - 0898-6568
IS - 1
ER -