Abstract
The role of ATP on regulation of the Na+/K+-ATPase activity in the human cancerous HeLa cells was investigated. HeLa cells stimulated with increasing ATP concentrations showed a dose-dependent inhibition of the Na+/K+-ATPase activity. These effects were also obtained by UTP. ATP and UTP provoked a rise in intracellular calcium concentration ([Ca2+]i) persisting for at least 4 min. The inhibitor of phospholipase C, U73122, blocked the elevation of [Ca2+]i provoked by ATP/UTP. The expression of mRNA for P2Y2 and P2Y6 receptors was demonstrated by RT-PCR. ATP/UTP activated PKC-α, -βI and -ε isoforms, but not PKC-δ and -ζ. The inhibition of the Na+/K+-ATPase activity by ATP/UTP was blocked by Gö6976, a specific inhibitor of the calcium-dependent PKCs. In conclusion, our results suggest that ATP/UTP modulate Na+/K+-ATPase activity in HeLa cells through the P2Y2 purinoceptor via calcium mobilisation and activation of calcium-dependent PKCs.
Original language | English |
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Pages (from-to) | 115-121 |
Number of pages | 7 |
Journal | Cellular Signalling |
Volume | 15 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 1 2003 |
Keywords
- ATP
- HeLa
- Na/K ATPase
ASJC Scopus subject areas
- Cell Biology