Activation of PKC-β isoforms mediates HNE-induced MCP-1 release by macrophages

M. Nitti, C. Domenicotti, C. D'Abramo, S. Assereto, D. Cottalasso, E. Melloni, G. Poli, F. Biasi, U. M. Marinari, M. A. Pronzato

Research output: Contribution to journalArticlepeer-review


4-Hydroxynonenal (HNE) in the concentration range detectable in many pathophysiologic conditions is able to modulate signal transduction cascades and gene expression. Here, we report the stimulating effect of 1 μM HNE on the release of the monocyte chemotactic protein-1 (MCP-1) by murine macrophages. MCP-1-increased export following 1-h cell treatment with HNE proved to be comparable to that exerted by standard amounts of bacterial lipopolysaccharide (LPS). However, the key molecular event in HNE-induced secretion of MCP-1 appeared to be the increased activity of β-PKC isoforms, which are recognized as playing a role in the regulation of cell protein transport and secretion. On the other hand, in LPS-stimulated cells, the δ isoform was seen to be involved and was probably related to LPS-mediated effects on MCP-1 expression and synthesis. In conclusion, HNE might interact with other pro-inflammatory stimuli, like LPS, in a concerted amplification of MCP-1 production and secretion.

Original languageEnglish
Pages (from-to)547-552
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 2002


  • 4-Hydroxynonenal
  • Cell signaling
  • Macrophages
  • MCP-1
  • PKC isoenzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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