Activation of PKC-β isoforms mediates HNE-induced MCP-1 release by macrophages

M. Nitti; C. Domenicotti; C. D'Abramo; S. Assereto; D. Cottalasso; E. Melloni; G. Poli; F. Biasi; U. M. Marinari; M. A. Pronzato

doi:10.1016/S0006-291X(02)00512-0

Activation of PKC-β isoforms mediates HNE-induced MCP-1 release by macrophages

M. Nitti, C. Domenicotti, C. D'Abramo, S. Assereto, D. Cottalasso, E. Melloni, G. Poli, F. Biasi, U. M. Marinari, M. A. Pronzato

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article

Abstract

4-Hydroxynonenal (HNE) in the concentration range detectable in many pathophysiologic conditions is able to modulate signal transduction cascades and gene expression. Here, we report the stimulating effect of 1 μM HNE on the release of the monocyte chemotactic protein-1 (MCP-1) by murine macrophages. MCP-1-increased export following 1-h cell treatment with HNE proved to be comparable to that exerted by standard amounts of bacterial lipopolysaccharide (LPS). However, the key molecular event in HNE-induced secretion of MCP-1 appeared to be the increased activity of β-PKC isoforms, which are recognized as playing a role in the regulation of cell protein transport and secretion. On the other hand, in LPS-stimulated cells, the δ isoform was seen to be involved and was probably related to LPS-mediated effects on MCP-1 expression and synthesis. In conclusion, HNE might interact with other pro-inflammatory stimuli, like LPS, in a concerted amplification of MCP-1 production and secretion.

Original language	English
Pages (from-to)	547-552
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	294
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(02)00512-0
Publication status	Published - 2002

Keywords

4-Hydroxynonenal
Cell signaling
Macrophages
MCP-1
PKC isoenzymes

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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Nitti, M., Domenicotti, C., D'Abramo, C., Assereto, S., Cottalasso, D., Melloni, E., Poli, G., Biasi, F., Marinari, U. M., & Pronzato, M. A. (2002). Activation of PKC-β isoforms mediates HNE-induced MCP-1 release by macrophages. Biochemical and Biophysical Research Communications, 294(3), 547-552. https://doi.org/10.1016/S0006-291X(02)00512-0

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abstract = "4-Hydroxynonenal (HNE) in the concentration range detectable in many pathophysiologic conditions is able to modulate signal transduction cascades and gene expression. Here, we report the stimulating effect of 1 μM HNE on the release of the monocyte chemotactic protein-1 (MCP-1) by murine macrophages. MCP-1-increased export following 1-h cell treatment with HNE proved to be comparable to that exerted by standard amounts of bacterial lipopolysaccharide (LPS). However, the key molecular event in HNE-induced secretion of MCP-1 appeared to be the increased activity of β-PKC isoforms, which are recognized as playing a role in the regulation of cell protein transport and secretion. On the other hand, in LPS-stimulated cells, the δ isoform was seen to be involved and was probably related to LPS-mediated effects on MCP-1 expression and synthesis. In conclusion, HNE might interact with other pro-inflammatory stimuli, like LPS, in a concerted amplification of MCP-1 production and secretion.",

keywords = "4-Hydroxynonenal, Cell signaling, Macrophages, MCP-1, PKC isoenzymes",

author = "M. Nitti and C. Domenicotti and C. D'Abramo and S. Assereto and D. Cottalasso and E. Melloni and G. Poli and F. Biasi and Marinari, {U. M.} and Pronzato, {M. A.}",

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T1 - Activation of PKC-β isoforms mediates HNE-induced MCP-1 release by macrophages

AU - Nitti, M.

AU - Domenicotti, C.

AU - D'Abramo, C.

AU - Assereto, S.

AU - Cottalasso, D.

AU - Melloni, E.

AU - Poli, G.

AU - Biasi, F.

AU - Marinari, U. M.

AU - Pronzato, M. A.

PY - 2002

Y1 - 2002

N2 - 4-Hydroxynonenal (HNE) in the concentration range detectable in many pathophysiologic conditions is able to modulate signal transduction cascades and gene expression. Here, we report the stimulating effect of 1 μM HNE on the release of the monocyte chemotactic protein-1 (MCP-1) by murine macrophages. MCP-1-increased export following 1-h cell treatment with HNE proved to be comparable to that exerted by standard amounts of bacterial lipopolysaccharide (LPS). However, the key molecular event in HNE-induced secretion of MCP-1 appeared to be the increased activity of β-PKC isoforms, which are recognized as playing a role in the regulation of cell protein transport and secretion. On the other hand, in LPS-stimulated cells, the δ isoform was seen to be involved and was probably related to LPS-mediated effects on MCP-1 expression and synthesis. In conclusion, HNE might interact with other pro-inflammatory stimuli, like LPS, in a concerted amplification of MCP-1 production and secretion.

AB - 4-Hydroxynonenal (HNE) in the concentration range detectable in many pathophysiologic conditions is able to modulate signal transduction cascades and gene expression. Here, we report the stimulating effect of 1 μM HNE on the release of the monocyte chemotactic protein-1 (MCP-1) by murine macrophages. MCP-1-increased export following 1-h cell treatment with HNE proved to be comparable to that exerted by standard amounts of bacterial lipopolysaccharide (LPS). However, the key molecular event in HNE-induced secretion of MCP-1 appeared to be the increased activity of β-PKC isoforms, which are recognized as playing a role in the regulation of cell protein transport and secretion. On the other hand, in LPS-stimulated cells, the δ isoform was seen to be involved and was probably related to LPS-mediated effects on MCP-1 expression and synthesis. In conclusion, HNE might interact with other pro-inflammatory stimuli, like LPS, in a concerted amplification of MCP-1 production and secretion.

KW - 4-Hydroxynonenal

KW - Cell signaling

KW - Macrophages

KW - MCP-1

KW - PKC isoenzymes

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