Abstract
4-Hydroxynonenal (HNE) in the concentration range detectable in many pathophysiologic conditions is able to modulate signal transduction cascades and gene expression. Here, we report the stimulating effect of 1 μM HNE on the release of the monocyte chemotactic protein-1 (MCP-1) by murine macrophages. MCP-1-increased export following 1-h cell treatment with HNE proved to be comparable to that exerted by standard amounts of bacterial lipopolysaccharide (LPS). However, the key molecular event in HNE-induced secretion of MCP-1 appeared to be the increased activity of β-PKC isoforms, which are recognized as playing a role in the regulation of cell protein transport and secretion. On the other hand, in LPS-stimulated cells, the δ isoform was seen to be involved and was probably related to LPS-mediated effects on MCP-1 expression and synthesis. In conclusion, HNE might interact with other pro-inflammatory stimuli, like LPS, in a concerted amplification of MCP-1 production and secretion.
| Original language | English |
|---|---|
| Pages (from-to) | 547-552 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 294 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2002 |
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Keywords
- 4-Hydroxynonenal
- Cell signaling
- Macrophages
- MCP-1
- PKC isoenzymes
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Activation of PKC-β isoforms mediates HNE-induced MCP-1 release by macrophages. / Nitti, M.; Domenicotti, C.; D'Abramo, C.; Assereto, S.; Cottalasso, D.; Melloni, E.; Poli, G.; Biasi, F.; Marinari, U. M.; Pronzato, M. A.
In: Biochemical and Biophysical Research Communications, Vol. 294, No. 3, 2002, p. 547-552.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Activation of PKC-β isoforms mediates HNE-induced MCP-1 release by macrophages
AU - Nitti, M.
AU - Domenicotti, C.
AU - D'Abramo, C.
AU - Assereto, S.
AU - Cottalasso, D.
AU - Melloni, E.
AU - Poli, G.
AU - Biasi, F.
AU - Marinari, U. M.
AU - Pronzato, M. A.
PY - 2002
Y1 - 2002
N2 - 4-Hydroxynonenal (HNE) in the concentration range detectable in many pathophysiologic conditions is able to modulate signal transduction cascades and gene expression. Here, we report the stimulating effect of 1 μM HNE on the release of the monocyte chemotactic protein-1 (MCP-1) by murine macrophages. MCP-1-increased export following 1-h cell treatment with HNE proved to be comparable to that exerted by standard amounts of bacterial lipopolysaccharide (LPS). However, the key molecular event in HNE-induced secretion of MCP-1 appeared to be the increased activity of β-PKC isoforms, which are recognized as playing a role in the regulation of cell protein transport and secretion. On the other hand, in LPS-stimulated cells, the δ isoform was seen to be involved and was probably related to LPS-mediated effects on MCP-1 expression and synthesis. In conclusion, HNE might interact with other pro-inflammatory stimuli, like LPS, in a concerted amplification of MCP-1 production and secretion.
AB - 4-Hydroxynonenal (HNE) in the concentration range detectable in many pathophysiologic conditions is able to modulate signal transduction cascades and gene expression. Here, we report the stimulating effect of 1 μM HNE on the release of the monocyte chemotactic protein-1 (MCP-1) by murine macrophages. MCP-1-increased export following 1-h cell treatment with HNE proved to be comparable to that exerted by standard amounts of bacterial lipopolysaccharide (LPS). However, the key molecular event in HNE-induced secretion of MCP-1 appeared to be the increased activity of β-PKC isoforms, which are recognized as playing a role in the regulation of cell protein transport and secretion. On the other hand, in LPS-stimulated cells, the δ isoform was seen to be involved and was probably related to LPS-mediated effects on MCP-1 expression and synthesis. In conclusion, HNE might interact with other pro-inflammatory stimuli, like LPS, in a concerted amplification of MCP-1 production and secretion.
KW - 4-Hydroxynonenal
KW - Cell signaling
KW - Macrophages
KW - MCP-1
KW - PKC isoenzymes
UR - http://www.scopus.com/inward/record.url?scp=0036297299&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036297299&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(02)00512-0
DO - 10.1016/S0006-291X(02)00512-0
M3 - Article
C2 - 12056801
AN - SCOPUS:0036297299
VL - 294
SP - 547
EP - 552
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -