Abstract
4-Hydroxynonenal (HNE) in the concentration range detectable in many pathophysiologic conditions is able to modulate signal transduction cascades and gene expression. Here, we report the stimulating effect of 1 μM HNE on the release of the monocyte chemotactic protein-1 (MCP-1) by murine macrophages. MCP-1-increased export following 1-h cell treatment with HNE proved to be comparable to that exerted by standard amounts of bacterial lipopolysaccharide (LPS). However, the key molecular event in HNE-induced secretion of MCP-1 appeared to be the increased activity of β-PKC isoforms, which are recognized as playing a role in the regulation of cell protein transport and secretion. On the other hand, in LPS-stimulated cells, the δ isoform was seen to be involved and was probably related to LPS-mediated effects on MCP-1 expression and synthesis. In conclusion, HNE might interact with other pro-inflammatory stimuli, like LPS, in a concerted amplification of MCP-1 production and secretion.
Original language | English |
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Pages (from-to) | 547-552 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 294 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2002 |
Keywords
- 4-Hydroxynonenal
- Cell signaling
- Macrophages
- MCP-1
- PKC isoenzymes
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology