Activation of PKC-β isoforms mediates HNE-induced MCP-1 release by macrophages

M. Nitti, C. Domenicotti, C. D'Abramo, S. Assereto, D. Cottalasso, E. Melloni, G. Poli, F. Biasi, U. M. Marinari, M. A. Pronzato

Research output: Contribution to journalArticle

Abstract

4-Hydroxynonenal (HNE) in the concentration range detectable in many pathophysiologic conditions is able to modulate signal transduction cascades and gene expression. Here, we report the stimulating effect of 1 μM HNE on the release of the monocyte chemotactic protein-1 (MCP-1) by murine macrophages. MCP-1-increased export following 1-h cell treatment with HNE proved to be comparable to that exerted by standard amounts of bacterial lipopolysaccharide (LPS). However, the key molecular event in HNE-induced secretion of MCP-1 appeared to be the increased activity of β-PKC isoforms, which are recognized as playing a role in the regulation of cell protein transport and secretion. On the other hand, in LPS-stimulated cells, the δ isoform was seen to be involved and was probably related to LPS-mediated effects on MCP-1 expression and synthesis. In conclusion, HNE might interact with other pro-inflammatory stimuli, like LPS, in a concerted amplification of MCP-1 production and secretion.

Original languageEnglish
Pages (from-to)547-552
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume294
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

Chemokine CCL2
Macrophages
Protein Isoforms
Chemical activation
Lipopolysaccharides
Signal transduction
Protein Transport
Gene expression
Amplification
Signal Transduction
Cells
4-hydroxy-2-nonenal
Gene Expression
Proteins

Keywords

  • 4-Hydroxynonenal
  • Cell signaling
  • Macrophages
  • MCP-1
  • PKC isoenzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Nitti, M., Domenicotti, C., D'Abramo, C., Assereto, S., Cottalasso, D., Melloni, E., ... Pronzato, M. A. (2002). Activation of PKC-β isoforms mediates HNE-induced MCP-1 release by macrophages. Biochemical and Biophysical Research Communications, 294(3), 547-552. https://doi.org/10.1016/S0006-291X(02)00512-0

Activation of PKC-β isoforms mediates HNE-induced MCP-1 release by macrophages. / Nitti, M.; Domenicotti, C.; D'Abramo, C.; Assereto, S.; Cottalasso, D.; Melloni, E.; Poli, G.; Biasi, F.; Marinari, U. M.; Pronzato, M. A.

In: Biochemical and Biophysical Research Communications, Vol. 294, No. 3, 2002, p. 547-552.

Research output: Contribution to journalArticle

Nitti, M, Domenicotti, C, D'Abramo, C, Assereto, S, Cottalasso, D, Melloni, E, Poli, G, Biasi, F, Marinari, UM & Pronzato, MA 2002, 'Activation of PKC-β isoforms mediates HNE-induced MCP-1 release by macrophages', Biochemical and Biophysical Research Communications, vol. 294, no. 3, pp. 547-552. https://doi.org/10.1016/S0006-291X(02)00512-0
Nitti, M. ; Domenicotti, C. ; D'Abramo, C. ; Assereto, S. ; Cottalasso, D. ; Melloni, E. ; Poli, G. ; Biasi, F. ; Marinari, U. M. ; Pronzato, M. A. / Activation of PKC-β isoforms mediates HNE-induced MCP-1 release by macrophages. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 294, No. 3. pp. 547-552.
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