Activation of PPARγ and δ by dietary punicic acid ameliorates intestinal inflammation in mice

Josep Bassaganya-Riera, Margaret DiGuardo, Montse Climent, Cristina Vives, Adria Carbo, Zeina E. Jouni, Alexandra W C Einerhand, Marianne O'Shea, Raquel Hontecillas

Research output: Contribution to journalArticlepeer-review


The goal of the present study was to elucidate the mechanisms of immunoregulation by which dietary punicic acid (PUA) prevents or ameliorates experimental inflammatory bowel disease (IBD). The expression of PPARγ and δ, their responsive genes and pro-inflammatory cytokines was assayed in the colonic mucosa. Immune cell-specific PPARγ null, PPARδ knockout and wild-type mice were treated with PUA and challenged with 2·5 % dextran sodium sulphate (DSS). The prophylactic efficacy of PUA was examined in an IL-10 -/- model of IBD. The effect of PUA on the regulatory T-cell (Treg) compartment was also examined in mice with experimental IBD. PUA ameliorated spontaneous pan-enteritis in IL-10 -/- mice and DSS colitis, up-regulated Foxp3 expression in Treg and suppressed TNF-α, but the loss of functional PPARγ or δ impaired these anti-inflammatory effects. At the cellular level, the macrophage-specific deletion of PPARγ caused a complete abrogation of the protective effect of PUA, whereas the deletion of PPARδ or intestinal epithelial cell-specific PPARγ decreased its anti-inflammatory efficacy. We provide in vivo molecular evidence demonstrating that PUA ameliorates experimental IBD by regulating macrophage and T-cell function through PPARγ- and δ-dependent mechanisms.

Original languageEnglish
Pages (from-to)878-886
Number of pages9
JournalBritish Journal of Nutrition
Issue number6
Publication statusPublished - Sep 28 2011


  • Inflammatory bowel disease
  • PPAR
  • Punicic acid

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Medicine(all)


Dive into the research topics of 'Activation of PPARγ and δ by dietary punicic acid ameliorates intestinal inflammation in mice'. Together they form a unique fingerprint.

Cite this