Activation of signal transducer and activator of transcription 3 in rat liver after heat shock and reperfusion stress

Lorenza Tacchini, Daniela Fusar-Poli, Marina Sironi, Alberto Mantovani, Aldo Bernelli-Zazzera

Research output: Contribution to journalArticle

Abstract

Changes in transcription factors (TFs) accompany many types of cell stresses. By using electrophoretic mobility assays we show that the DNA binding of signal transducer and activator of transcription 3 (STAT3) is activated in rat liver by heat shock and ischemia-reperfusion. Northern blot and Western blot analysis reveal an increase of the mRNA and protein level of this transcription factor. Under both conditions the phosphorylation of pre-existing STAT3 is prompt and precedes the increase in the STAT3 protein. The activation: (1) is functional, i.e. is followed by the transcription of the target gene α1-acid glycoprotein (2) is strongly inhibited by pretreatment with the interleukin-1 receptor antagonist before heat shock but only slightly by pretreatment before ischemia-reperfusion (3) might, at least in part, be mediated by a cytokine cascade involving also interleukin-6. These results are consistent with the hypothesis that different kinds of stress can activate a number of common TFs.

Original languageEnglish
Pages (from-to)316-323
Number of pages8
JournalInternational Journal of Biochemistry and Cell Biology
Volume35
Issue number3
DOIs
Publication statusPublished - Mar 1 2003

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Keywords

  • Cytokines
  • Heat shock
  • Ischemia/reperfusion
  • Liver
  • Transcription factors (TFs)

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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