TY - JOUR
T1 - Activation-Related Differences in HLA Class I-Bound Peptides
T2 - Presentation of an IL-1 Receptor Antagonist-Derived Peptide by Activated, but Not Resting, CD4+ T Lymphocytes
AU - Frumento, Guido
AU - Corradi, Anna
AU - Ferrara, Giovanni B.
AU - Rubartelli, Anna
PY - 1997/12/15
Y1 - 1997/12/15
N2 - We have compared by reverse phase HPLC the set of peptides eluted from HLA class I molecules in resting and activated CD4+ Jurkat cells. Two peptides were identified that are presented de novo upon activation. After sequencing, one of these peptides turned out to derive from IL-1R antagonist (IL-1Ra). In keeping with this observation, we found that activated, but not resting, Jurkat cells express IL-1Ra. These data indicate that activation of a CD4+ T cell line may result in presentation of peptides derived from proteins expressed de novo after activation. Since IL-1 Ra was not known to be expressed by cells of the T lineage, we also investigated its pattern of expression in normal T lymphocytes. Reverse transcriptase-PCR analyses allowed us to demonstrate that IL-1Ra is expressed upon activation by normal CD4+ lymphocytes from peripheral blood and by thymocytes, but not by CD8+ T cells. Interestingly, of the two forms of IL-1Ra that have been detected in different cell lineages, the intracellular one and the secreted one, only the former is expressed by activated CD4+ T cells.
AB - We have compared by reverse phase HPLC the set of peptides eluted from HLA class I molecules in resting and activated CD4+ Jurkat cells. Two peptides were identified that are presented de novo upon activation. After sequencing, one of these peptides turned out to derive from IL-1R antagonist (IL-1Ra). In keeping with this observation, we found that activated, but not resting, Jurkat cells express IL-1Ra. These data indicate that activation of a CD4+ T cell line may result in presentation of peptides derived from proteins expressed de novo after activation. Since IL-1 Ra was not known to be expressed by cells of the T lineage, we also investigated its pattern of expression in normal T lymphocytes. Reverse transcriptase-PCR analyses allowed us to demonstrate that IL-1Ra is expressed upon activation by normal CD4+ lymphocytes from peripheral blood and by thymocytes, but not by CD8+ T cells. Interestingly, of the two forms of IL-1Ra that have been detected in different cell lineages, the intracellular one and the secreted one, only the former is expressed by activated CD4+ T cells.
UR - http://www.scopus.com/inward/record.url?scp=0031573547&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031573547&partnerID=8YFLogxK
M3 - Article
C2 - 9550397
AN - SCOPUS:0031573547
VL - 159
SP - 5993
EP - 5999
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 12
ER -