Active caspase-3 detection to evaluate apoptosis induced by Verbena officinalis essential oil and citral in chronic lymphocytic leukaemia cells

Laura de Martino, Giovanni D. Arena, Maria Marta Minervini, Silvia Deaglio, Nicola Pio Sinisi, Nicola Cascavilla, Vincenzo de Feo

Research output: Contribution to journalArticlepeer-review

Abstract

Verbena officinalis L., Verbenaceae, commonly known as vervain, is a plant widely used in medicine. Despite of its widespread use in different traditional practices, the mechanisms of pharmacological actions of the plant and its volatile oil are still unclear We evaluated the pro-apoptotic activity of V officinalis essential oil and of its main component, citral, on lymphocytes collected from ten patients with chronic lymphocytic leukaemia (CLL), a disease in which a faulty apoptotic mechanism is still retained one of the primary pathogenic events, by adding to treated mononuclear cells, annexin-V, propidium iodide, and CD19 Apoptosis was also evaluated using anti-active-caspase-3 monoclonal antibody after permeabilization of the cells Both V.officinalis essential oil and citral were found able to induce apoptosis in CLL cells and to activate caspase-3, which is considered the way by means they active apoptosis in B neoplastic cells This data further support evidences that indicate natural compounds as possible lead structure to develop new therapeutic agents for CLL.

Original languageEnglish
Pages (from-to)869-873
Number of pages5
JournalBrazilian Journal of Pharmacognosy
Volume21
Issue number5
DOIs
Publication statusPublished - Sep 2011

Keywords

  • Apoptosis
  • Caspase-3
  • Chronic lymphocytic leukaemia
  • Citral
  • Flow cytometry
  • Verbena officinalis

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)

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