TY - JOUR
T1 - Active caspase-3 detection to evaluate apoptosis induced by Verbena officinalis essential oil and citral in chronic lymphocytic leukaemia cells
AU - de Martino, Laura
AU - Arena, Giovanni D.
AU - Minervini, Maria Marta
AU - Deaglio, Silvia
AU - Sinisi, Nicola Pio
AU - Cascavilla, Nicola
AU - de Feo, Vincenzo
PY - 2011/9
Y1 - 2011/9
N2 - Verbena officinalis L., Verbenaceae, commonly known as vervain, is a plant widely used in medicine. Despite of its widespread use in different traditional practices, the mechanisms of pharmacological actions of the plant and its volatile oil are still unclear We evaluated the pro-apoptotic activity of V officinalis essential oil and of its main component, citral, on lymphocytes collected from ten patients with chronic lymphocytic leukaemia (CLL), a disease in which a faulty apoptotic mechanism is still retained one of the primary pathogenic events, by adding to treated mononuclear cells, annexin-V, propidium iodide, and CD19 Apoptosis was also evaluated using anti-active-caspase-3 monoclonal antibody after permeabilization of the cells Both V.officinalis essential oil and citral were found able to induce apoptosis in CLL cells and to activate caspase-3, which is considered the way by means they active apoptosis in B neoplastic cells This data further support evidences that indicate natural compounds as possible lead structure to develop new therapeutic agents for CLL.
AB - Verbena officinalis L., Verbenaceae, commonly known as vervain, is a plant widely used in medicine. Despite of its widespread use in different traditional practices, the mechanisms of pharmacological actions of the plant and its volatile oil are still unclear We evaluated the pro-apoptotic activity of V officinalis essential oil and of its main component, citral, on lymphocytes collected from ten patients with chronic lymphocytic leukaemia (CLL), a disease in which a faulty apoptotic mechanism is still retained one of the primary pathogenic events, by adding to treated mononuclear cells, annexin-V, propidium iodide, and CD19 Apoptosis was also evaluated using anti-active-caspase-3 monoclonal antibody after permeabilization of the cells Both V.officinalis essential oil and citral were found able to induce apoptosis in CLL cells and to activate caspase-3, which is considered the way by means they active apoptosis in B neoplastic cells This data further support evidences that indicate natural compounds as possible lead structure to develop new therapeutic agents for CLL.
KW - Apoptosis
KW - Caspase-3
KW - Chronic lymphocytic leukaemia
KW - Citral
KW - Flow cytometry
KW - Verbena officinalis
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U2 - 10.1590/S0102-695X2011005000082
DO - 10.1590/S0102-695X2011005000082
M3 - Article
AN - SCOPUS:80053447117
VL - 21
SP - 869
EP - 873
JO - Revista Brasileira de Farmacognosia
JF - Revista Brasileira de Farmacognosia
SN - 0102-695X
IS - 5
ER -