TY - JOUR
T1 - Active localization of the retinoblastoma protein in chromatin and its response to S phase DNA damage
AU - Avni, Dror
AU - Yang, Hong
AU - Martelli, Fabio
AU - Hofmann, Francesco
AU - ElShamy, Wael M.
AU - Ganesan, Shridar
AU - Scully, Ralph
AU - Livingston, David M.
PY - 2003/9/1
Y1 - 2003/9/1
N2 - The Rb protein suppresses development of an abnormal state of endoreduplication arising after S phase DNA damage. In diploid, S phase cells, the activity of protein phosphatase 2A (PP2A) licenses the stable association of un(der)phosphorylated Rb with chromatin. After damage, chromatin-associated pRb is attracted to certain chromosomal replication initiation sites in the order in which they normally fire. Like S phase DNA damage in Rb-/- cells, specific interruption of PP2A function in irradiated, S phase wt cells also elicited a state of endoreduplication. Thus, PP2A normally licenses the recruitment of Rb to chromatin sites in S phase from which, after DNA damage, it relocalizes to selected replication control sites and suppresses abnormal, postdamage rereplicative activity.
AB - The Rb protein suppresses development of an abnormal state of endoreduplication arising after S phase DNA damage. In diploid, S phase cells, the activity of protein phosphatase 2A (PP2A) licenses the stable association of un(der)phosphorylated Rb with chromatin. After damage, chromatin-associated pRb is attracted to certain chromosomal replication initiation sites in the order in which they normally fire. Like S phase DNA damage in Rb-/- cells, specific interruption of PP2A function in irradiated, S phase wt cells also elicited a state of endoreduplication. Thus, PP2A normally licenses the recruitment of Rb to chromatin sites in S phase from which, after DNA damage, it relocalizes to selected replication control sites and suppresses abnormal, postdamage rereplicative activity.
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U2 - 10.1016/S1097-2765(03)00355-1
DO - 10.1016/S1097-2765(03)00355-1
M3 - Article
C2 - 14527418
AN - SCOPUS:0141861977
VL - 12
SP - 735
EP - 746
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 3
ER -