Active-site titration of serine proteinases acting selectively on cationic substrates by N-α-carbobenzoxy-l-arginine p-nitrophenyl ester and N-α-carbobenzoxy-l-lysine p-nitrophenyl ester; determination of active enzyme concentration

Paolo Ascenzi, Enea Menegatti, Mario Guarneri, Gino Amiconi

Research output: Contribution to journalArticle

Abstract

A titration method for determination of trypsin-like serine proteinase concentration has been developed by using ZArgONp and ZLysONp, two specific chromogenic amino-acid derivatives which show the characteristics of optimal active-site titrants. Active proteinase concentration has been estimated from the effect of titrant concentration on the amplitude, at time zero, of the time-course for the instantaneous release of p-nitrophenol, preceding the steady-state reaction (burst phase).

Original languageEnglish
Pages (from-to)421-425
Number of pages5
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume915
Issue number3
DOIs
Publication statusPublished - Oct 15 1987

Keywords

  • Active enzyme concentration
  • Active site titrant
  • Enzyme kinetics
  • Serine proteinase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Structural Biology

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