Active thymopoiesis in idiopathic chronic pancreatitis

R. Cianci, C. Spada, V. Perri, G. Cammarota, R. Urgesi, S. Lolli, E. Girardi, G. Costamagna, Franco Pandolfi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background/Aims: Cellular immunity has a pivotal role in the pathogenesis of chronic pancreatitis (CP), resulting in pancreas infiltration by T-cells. Studies on systemic immunity are few and contradictory. One study reported a decrease of naive CD45RA+ cells. The presence of naive T cells, detected as recent thymic emigrants (RTEs), is evaluated with a new molecular technique by using real-time PCR to detect the T-cell receptor excision circles (TREC). To elucidate the role of naive T-cells in the pathogenesis of CP, we investigated the percentage of sj-TREC in CP patients. Patients: Thirty CP patients were studied and compared to 30 sex- and age-matched healthy volunteers. Methods: Genomic DNA was isolated from peripheral blood mononuclear cells (PBMC) of each patient. RTEs were evaluated by measuring sj-TREC by real-time PCR. Results: The mean percentage of sj-TREC+ cells present in CP was not significantly different from that of control group (0.02319% vs 0.02338%, respectively). Conclusion: Our data show that naive TREC+ cells are normally represented in CR The presence of active thymopoiesis may be the underlying mechanism resulting in continuous production of T-cells, responsible of maintaining the inflammatory process.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalEuropean Review for Medical and Pharmacological Sciences
Volume12
Issue number1
Publication statusPublished - Jan 2008

Fingerprint

Chronic Pancreatitis
T-Cell Antigen Receptor
T-Lymphocytes
Real-Time Polymerase Chain Reaction
Cellular Immunity
Pancreas
Immunity
Blood Cells
Healthy Volunteers
Control Groups
DNA

Keywords

  • Chronic Pancreatitis (CP)
  • Recent Thymic Emigrants (RTEs)
  • Signal joint-TREC (sj-TREC)
  • T-cell Receptor Excision Circles (TREC)

ASJC Scopus subject areas

  • Pharmacology

Cite this

Cianci, R., Spada, C., Perri, V., Cammarota, G., Urgesi, R., Lolli, S., ... Pandolfi, F. (2008). Active thymopoiesis in idiopathic chronic pancreatitis. European Review for Medical and Pharmacological Sciences, 12(1), 41-46.

Active thymopoiesis in idiopathic chronic pancreatitis. / Cianci, R.; Spada, C.; Perri, V.; Cammarota, G.; Urgesi, R.; Lolli, S.; Girardi, E.; Costamagna, G.; Pandolfi, Franco.

In: European Review for Medical and Pharmacological Sciences, Vol. 12, No. 1, 01.2008, p. 41-46.

Research output: Contribution to journalArticle

Cianci, R, Spada, C, Perri, V, Cammarota, G, Urgesi, R, Lolli, S, Girardi, E, Costamagna, G & Pandolfi, F 2008, 'Active thymopoiesis in idiopathic chronic pancreatitis', European Review for Medical and Pharmacological Sciences, vol. 12, no. 1, pp. 41-46.
Cianci R, Spada C, Perri V, Cammarota G, Urgesi R, Lolli S et al. Active thymopoiesis in idiopathic chronic pancreatitis. European Review for Medical and Pharmacological Sciences. 2008 Jan;12(1):41-46.
Cianci, R. ; Spada, C. ; Perri, V. ; Cammarota, G. ; Urgesi, R. ; Lolli, S. ; Girardi, E. ; Costamagna, G. ; Pandolfi, Franco. / Active thymopoiesis in idiopathic chronic pancreatitis. In: European Review for Medical and Pharmacological Sciences. 2008 ; Vol. 12, No. 1. pp. 41-46.
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