Active thymopoiesis in idiopathic chronic pancreatitis

R. Cianci, C. Spada, V. Perri, G. Cammarota, R. Urgesi, S. Lolli, E. Girardi, G. Costamagna, Franco Pandolfi

Research output: Contribution to journalArticle

Abstract

Background/Aims: Cellular immunity has a pivotal role in the pathogenesis of chronic pancreatitis (CP), resulting in pancreas infiltration by T-cells. Studies on systemic immunity are few and contradictory. One study reported a decrease of naive CD45RA+ cells. The presence of naive T cells, detected as recent thymic emigrants (RTEs), is evaluated with a new molecular technique by using real-time PCR to detect the T-cell receptor excision circles (TREC). To elucidate the role of naive T-cells in the pathogenesis of CP, we investigated the percentage of sj-TREC in CP patients. Patients: Thirty CP patients were studied and compared to 30 sex- and age-matched healthy volunteers. Methods: Genomic DNA was isolated from peripheral blood mononuclear cells (PBMC) of each patient. RTEs were evaluated by measuring sj-TREC by real-time PCR. Results: The mean percentage of sj-TREC+ cells present in CP was not significantly different from that of control group (0.02319% vs 0.02338%, respectively). Conclusion: Our data show that naive TREC+ cells are normally represented in CR The presence of active thymopoiesis may be the underlying mechanism resulting in continuous production of T-cells, responsible of maintaining the inflammatory process.

Original languageEnglish
Pages (from-to)41-46
Number of pages6
JournalEuropean Review for Medical and Pharmacological Sciences
Volume12
Issue number1
Publication statusPublished - Jan 2008

Keywords

  • Chronic Pancreatitis (CP)
  • Recent Thymic Emigrants (RTEs)
  • Signal joint-TREC (sj-TREC)
  • T-cell Receptor Excision Circles (TREC)

ASJC Scopus subject areas

  • Pharmacology

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    Cianci, R., Spada, C., Perri, V., Cammarota, G., Urgesi, R., Lolli, S., Girardi, E., Costamagna, G., & Pandolfi, F. (2008). Active thymopoiesis in idiopathic chronic pancreatitis. European Review for Medical and Pharmacological Sciences, 12(1), 41-46.