Activin A induces langerhans cell differentiation in vitro and in human skin explants

Tiziana Musso, Sara Scutera, William Vermi, Roberta Daniele, Michele Fornaro, Carlotta Castagnoli, Daniela Alotto, Maria Ravanini, Irene Cambieri, Laura Salogni, Angela Rita Elia, Mirella Giovarelli, Fabio Facchetti, Giampiero Girolomoni, Silvano Sozzani

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Langerhans cells (LC) represent a well characterized subset of dendritic cells located in the epidermis of skin and mucosae. In vivo, they originate from resident and blood-borne precursors in the presence of keratinocyte-derived TGFβ. In vitro, LC can be generated from monocytes in the presence of GM-CSF, IL-4 and TGFβ. However, the signals that induce LC during an inflammatory reaction are not fully investigated. Here we report that Activin A, a TGFβ family member induced by proinflammatory cytokines and involved in skin morphogenesis and wound healing, induces the differentiation of human monocytes into LC in the absence of TGFβ. Activin A-induced LC are Langerin+, Birbeck granules+, E-cadherin+, CLA+ and CCR6+ and possess typical APC functions. In human skin explants, intradermal injection of Activin A increased the number of CD1a+ and Langerin+ cells in both the epidermis and dermis by promoting the differentiation of resident precursor cells. High levels of Activin A were present in the upper epidermal layers and in the dermis of Lichen Planus biopsies in association with a marked infiltration of CD1a+ and Langerin+ cells. This study reports that Activin A induces the differentiation of circulating CD14+ cells into LC. Since Activin A is abundantly produced during inflammatory conditions which are also characterized by increased numbers of LC, we propose that this cytokine represents a new pathway, alternative to TGFβ, responsible for LC differentiation during inflammatory/autoimmune conditions.

Original languageEnglish
Article numbere3271
JournalPLoS One
Volume3
Issue number9
DOIs
Publication statusPublished - Sep 24 2008

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Langerhans cells
activins
Langerhans Cells
skin (animal)
cell differentiation
Cell Differentiation
Skin
explants
dermis
epidermis (animal)
Dermis
Epidermis
monocytes
Cytokines
Monocytes
cytokines
Biopsy
cells
Cadherins
Granulocyte-Macrophage Colony-Stimulating Factor

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Musso, T., Scutera, S., Vermi, W., Daniele, R., Fornaro, M., Castagnoli, C., ... Sozzani, S. (2008). Activin A induces langerhans cell differentiation in vitro and in human skin explants. PLoS One, 3(9), [e3271]. https://doi.org/10.1371/journal.pone.0003271

Activin A induces langerhans cell differentiation in vitro and in human skin explants. / Musso, Tiziana; Scutera, Sara; Vermi, William; Daniele, Roberta; Fornaro, Michele; Castagnoli, Carlotta; Alotto, Daniela; Ravanini, Maria; Cambieri, Irene; Salogni, Laura; Elia, Angela Rita; Giovarelli, Mirella; Facchetti, Fabio; Girolomoni, Giampiero; Sozzani, Silvano.

In: PLoS One, Vol. 3, No. 9, e3271, 24.09.2008.

Research output: Contribution to journalArticle

Musso, T, Scutera, S, Vermi, W, Daniele, R, Fornaro, M, Castagnoli, C, Alotto, D, Ravanini, M, Cambieri, I, Salogni, L, Elia, AR, Giovarelli, M, Facchetti, F, Girolomoni, G & Sozzani, S 2008, 'Activin A induces langerhans cell differentiation in vitro and in human skin explants', PLoS One, vol. 3, no. 9, e3271. https://doi.org/10.1371/journal.pone.0003271
Musso T, Scutera S, Vermi W, Daniele R, Fornaro M, Castagnoli C et al. Activin A induces langerhans cell differentiation in vitro and in human skin explants. PLoS One. 2008 Sep 24;3(9). e3271. https://doi.org/10.1371/journal.pone.0003271
Musso, Tiziana ; Scutera, Sara ; Vermi, William ; Daniele, Roberta ; Fornaro, Michele ; Castagnoli, Carlotta ; Alotto, Daniela ; Ravanini, Maria ; Cambieri, Irene ; Salogni, Laura ; Elia, Angela Rita ; Giovarelli, Mirella ; Facchetti, Fabio ; Girolomoni, Giampiero ; Sozzani, Silvano. / Activin A induces langerhans cell differentiation in vitro and in human skin explants. In: PLoS One. 2008 ; Vol. 3, No. 9.
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