Activity-dependent phosphorylation of Ser187 is required for SNAP-25-negative modulation of neuronal voltage-gated calcium channels

Davide Pozzi, Steven Condliffe, Yuri Bozzi, Maia Chikhladze, Carlotta Grumelli, Véronique Proux-Gillardeaux, Masami Takahashi, Silvana Franceschetti, Claudia Verderio, Michela Matteoli

Research output: Contribution to journalArticle

Abstract

Synaptosomal-associated protein of 25 kDa (SNAP-25) is a SNARE protein that regulates neurotransmission by the formation of a complex with syntaxin 1 and synaptobrevin/VAMP2. SNAP-25 also reduces neuronal calcium responses to stimuli, but neither the functional relevance nor the molecular mechanisms of this modulation have been clarified. In this study, we demonstrate that hippocampal slices from Snap25+/- mice display a significantly larger facilitation and that higher calcium peaks are reached after depolarization by Snap25-/- and Snap25+/- cultured neurons compared with wild type. We also show that SNAP-25b modulates calcium dynamics by inhibiting voltage-gated calcium channels (VGCCs) and that PKC phosphorylation of SNAP-25 at ser187 is essential for this process, as indicated by the use of phosphomimetic (S187E) or nonphosphorylated (S187A) mutants. Neuronal activity is the trigger that induces the transient phosphorylation of SNAP-25 at ser187. Indeed, enhancement of network activity increases the levels of phosphorylated SNAP-25, whereas network inhibition reduces the extent of protein phosphorylation. A transient peak of SNAP-25 phosphorylation also is detectable in rat hippocampus in vivo after i.p. injection with kainate to induce seizures. These findings demonstrate that differences in the expression levels of SNAP-25 impact on calcium dynamics and neuronal plasticity, and that SNAP-25 phosphorylation, by promoting inhibition of VGCCs, may mediate a negative feedback modulation of neuronal activity during intense activation.

Original languageEnglish
Pages (from-to)323-328
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number1
DOIs
Publication statusPublished - Jan 8 2008

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Synaptosomal-Associated Protein 25
Calcium Channels
Phosphorylation
Calcium
Vesicle-Associated Membrane Protein 2
Syntaxin 1
R-SNARE Proteins
SNARE Proteins
Neuronal Plasticity
Kainic Acid
Synaptic Transmission
Hippocampus
Seizures
Neurons

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Activity-dependent phosphorylation of Ser187 is required for SNAP-25-negative modulation of neuronal voltage-gated calcium channels. / Pozzi, Davide; Condliffe, Steven; Bozzi, Yuri; Chikhladze, Maia; Grumelli, Carlotta; Proux-Gillardeaux, Véronique; Takahashi, Masami; Franceschetti, Silvana; Verderio, Claudia; Matteoli, Michela.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 1, 08.01.2008, p. 323-328.

Research output: Contribution to journalArticle

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