Activity in vitro of resveratrol on granulocyte and monocyte adhesion to endothelium

Maria Elena Ferrero, Alberto A E Bertelli, Alessandro Fulgenzi, Fabio Pellegatta, Massimiliano M. Corsi, Mimma Bonfrate, Franca Ferrara, Raffaele De Caterina, Luca Giovannini, Aldo Bertelli

Research output: Contribution to journalArticle

Abstract

Background: Resveratrol is a phytoalexin present in red wine. It has been shown to protect LDL from peroxidative degradation. Objective: In consideration of the low plasma concentration of orally adsorbed resveratrol (which is insufficient for antioxidant protection of LDL), we studied another effect of the compound. Design: Because resveratrol is a tyrosine kinase inhibitor like other members of the tyrphostin family, we hypothesized that it has the ability to modify intracellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) expression by stimulated endothelial cells. We studied the ability of resveratrol to inhibit such adhesion molecule expression and to block the adhesion of monocytes and granulocytes to endothelial cells. Results: We showed that resveratrol, at concentrations as low as 1 μmol/L and 100 nmol/L, significantly inhibited ICAM-1 and VCAM-1 expression by tumor necrosis factor α (TNF-α)-stimulated human umbilical vein endothelial cells and lipopolysaccharide-stimulated human saphenous vein endothelial cells (HSVEC), respectively. In addition, we showed that resveratrol induced a significant inhibition in the adhesion of U937 monocytoid cells to lipopolysaccharide-stimulated HSVEC. Such inhibition was comparable with that obtained when anti-VCAM-1 monoclonal antibody was used instead of resveratrol. Resveratrol also significantly inhibited the adhesion of neutrophils to TNF-α-stimulated NIH/3T3 ICAM-1-transfected cells, whereas neutrophils activated by formyl-methionyl-leucyl-phenylalanine did not significantly modify adhesion to NIH/3T3 ICAM-1-transfected cells. Conclusions: Our results indicate activity of resveratrol on endothelial cells and a new interpretation of an effect independent of its antioxidant function.

Original languageEnglish
Pages (from-to)1208-1214
Number of pages7
JournalAmerican Journal of Clinical Nutrition
Volume68
Issue number6
Publication statusPublished - Dec 1998

Fingerprint

resveratrol
granulocytes
endothelium
Granulocytes
monocytes
Endothelium
adhesion
Monocytes
endothelial cells
Endothelial Cells
Vascular Cell Adhesion Molecule-1
cell adhesion
blood vessels
saphenous vein
Aptitude
tumor necrosis factors
Saphenous Vein
lipopolysaccharides
Lipopolysaccharides
neutrophils

Keywords

  • Endothelium
  • Granulocytes
  • Grapes
  • LDL
  • Lipid peroxidation
  • Monocytes
  • Regulation of expression
  • Resveratrol
  • Wine

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Ferrero, M. E., Bertelli, A. A. E., Fulgenzi, A., Pellegatta, F., Corsi, M. M., Bonfrate, M., ... Bertelli, A. (1998). Activity in vitro of resveratrol on granulocyte and monocyte adhesion to endothelium. American Journal of Clinical Nutrition, 68(6), 1208-1214.

Activity in vitro of resveratrol on granulocyte and monocyte adhesion to endothelium. / Ferrero, Maria Elena; Bertelli, Alberto A E; Fulgenzi, Alessandro; Pellegatta, Fabio; Corsi, Massimiliano M.; Bonfrate, Mimma; Ferrara, Franca; De Caterina, Raffaele; Giovannini, Luca; Bertelli, Aldo.

In: American Journal of Clinical Nutrition, Vol. 68, No. 6, 12.1998, p. 1208-1214.

Research output: Contribution to journalArticle

Ferrero, ME, Bertelli, AAE, Fulgenzi, A, Pellegatta, F, Corsi, MM, Bonfrate, M, Ferrara, F, De Caterina, R, Giovannini, L & Bertelli, A 1998, 'Activity in vitro of resveratrol on granulocyte and monocyte adhesion to endothelium', American Journal of Clinical Nutrition, vol. 68, no. 6, pp. 1208-1214.
Ferrero ME, Bertelli AAE, Fulgenzi A, Pellegatta F, Corsi MM, Bonfrate M et al. Activity in vitro of resveratrol on granulocyte and monocyte adhesion to endothelium. American Journal of Clinical Nutrition. 1998 Dec;68(6):1208-1214.
Ferrero, Maria Elena ; Bertelli, Alberto A E ; Fulgenzi, Alessandro ; Pellegatta, Fabio ; Corsi, Massimiliano M. ; Bonfrate, Mimma ; Ferrara, Franca ; De Caterina, Raffaele ; Giovannini, Luca ; Bertelli, Aldo. / Activity in vitro of resveratrol on granulocyte and monocyte adhesion to endothelium. In: American Journal of Clinical Nutrition. 1998 ; Vol. 68, No. 6. pp. 1208-1214.
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abstract = "Background: Resveratrol is a phytoalexin present in red wine. It has been shown to protect LDL from peroxidative degradation. Objective: In consideration of the low plasma concentration of orally adsorbed resveratrol (which is insufficient for antioxidant protection of LDL), we studied another effect of the compound. Design: Because resveratrol is a tyrosine kinase inhibitor like other members of the tyrphostin family, we hypothesized that it has the ability to modify intracellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) expression by stimulated endothelial cells. We studied the ability of resveratrol to inhibit such adhesion molecule expression and to block the adhesion of monocytes and granulocytes to endothelial cells. Results: We showed that resveratrol, at concentrations as low as 1 μmol/L and 100 nmol/L, significantly inhibited ICAM-1 and VCAM-1 expression by tumor necrosis factor α (TNF-α)-stimulated human umbilical vein endothelial cells and lipopolysaccharide-stimulated human saphenous vein endothelial cells (HSVEC), respectively. In addition, we showed that resveratrol induced a significant inhibition in the adhesion of U937 monocytoid cells to lipopolysaccharide-stimulated HSVEC. Such inhibition was comparable with that obtained when anti-VCAM-1 monoclonal antibody was used instead of resveratrol. Resveratrol also significantly inhibited the adhesion of neutrophils to TNF-α-stimulated NIH/3T3 ICAM-1-transfected cells, whereas neutrophils activated by formyl-methionyl-leucyl-phenylalanine did not significantly modify adhesion to NIH/3T3 ICAM-1-transfected cells. Conclusions: Our results indicate activity of resveratrol on endothelial cells and a new interpretation of an effect independent of its antioxidant function.",
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AU - Ferrero, Maria Elena

AU - Bertelli, Alberto A E

AU - Fulgenzi, Alessandro

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AU - Corsi, Massimiliano M.

AU - Bonfrate, Mimma

AU - Ferrara, Franca

AU - De Caterina, Raffaele

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AU - Bertelli, Aldo

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