Activity levels of a β1,6 N-acetylglucosaminyltransferase in lymphomonocytes from multiple sclerosis patients

Antonio Orlacchio, Paola Sarchielli, Virgilio Gallai, Alessandro Datti, Carla Saccardi, Carlo A. Palmerini

Research output: Contribution to journalArticlepeer-review

Abstract

The activity of the Golgi glycosyltransferase β1,6 N- acetylglucosaminyltransferase (core 2 GlcNAc-T), which plays a role in T- cell activation and cell-cell adhesion, appears to be modulated in resting lymphomonocytes during different phases of multiple sclerosis (MS). In particular a significant decrease (25-30%) of the enzyme activity was observed with respect to healthy subjects in MS patients who were in relapse or in the very early stages of remission. A similar trend was found to be associated with patients affected by active lesions. A statistically significant decrease in the enzyme activity was also observed in patients with the progressive form. By contrast, core 2 GlcNAc-T activity did not appear correlated with duration of the disease. Interestingly, MS individuals under treatment with IFN-β1a, an immunosuppressive agent, showed levels of activity which were comparable with those observed in healthy subjects. Together, these observations suggest that down-regulation of core 2 GlcNAc-T activity is linked to the occurrence of acute phases in the relapsing- remitting form and to the progressive form of the disease, probably caused by altered expression of glycoproteins which are involved in lymphomonocyte activation and/or interaction with the endothelium. Additionally, it appears that the enzyme assay may provide a useful marker of the disease activity and the effects of therapeutical approaches.

Original languageEnglish
Pages (from-to)177-183
Number of pages7
JournalJournal of the Neurological Sciences
Volume151
Issue number2
DOIs
Publication statusPublished - Oct 22 1997

Keywords

  • Enzyme assay
  • Lymphomonocytes
  • Multiple sclerosis
  • N- acetylglucosaminyltransferase

ASJC Scopus subject areas

  • Ageing
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology

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