Activity of N-acetyl-L-cysteine against biofilm of Staphylococcus aureus and Pseudomonas aeruginosa on orthopedic prosthetic materials

Lorenzo Drago, Elena De Vecchi, Roberto Mattina, Carlo L. Romanò

Research output: Contribution to journalArticle

Abstract

Purpose: Staphylococcus aureus and Pseudomonas aeruginosa are frequently isolated from biofilm-associated orthopedic implant infections. This study assessed the activity of N-acetyl-L-cysteine at inhibitory and subinhibitory concentrations against biofilm formed by S. aureus and P. aeruginosa on prosthetic materials. Methods: S. aureus and P. aeruginosa biofilm on smooth polyethylene and sand-blasted titanium discs was determined by means of a spectrophotometric assay before and at 3, 6, and 18 hours after addition of N-acetyl-L-cysteine. Results: N-acetyl-L-cysteine was able to disaggregate biofilm produced by S. aureus and P. aerugi-nosa on polyethylene and titanium discs after 3 h of incubation. A different rate in eradication of biofilm produced by P. aeruginosa was seen between polyethylene and titanium (50% vs. 20% respectively vs. biofilm formed before treatment). Results were confirmed by confocal laser scanning microscopy. Conclusions: The results suggest a possible role of N-acetyl-L-cysteine in the treatment of orthopedic prosthetic infections.

Original languageEnglish
Pages (from-to)39-46
Number of pages8
JournalInternational Journal of Artificial Organs
Volume36
Issue number1
DOIs
Publication statusPublished - 2013

Keywords

  • Bacterial biofilm
  • N-acetyl-L-cysteine
  • Prosthetic joint infections
  • Pseudomonas aeruginosa
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

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