Acute and chronic saturated fatty acid treatment as a key instigator of the TLR-mediated inflammatory response in human adipose tissue, in vitro

Elham M. Youssef-Elabd, Kirsty C. McGee, Gyanendra Tripathi, Nasser Aldaghri, Mohga S. Abdalla, Hayat M. Sharada, Esmat Ashour, Ashraf I. Amin, Antonio Ceriello, Joseph P. O'Hare, Sudhesh Kumar, Philip G. McTernan, Alison L. Harte

Research output: Contribution to journalArticlepeer-review

Abstract

A post-prandial increase in saturated fatty acids (SFAs) and glucose (Glc) activates an inflammatory response, which may be prolonged following restoration of physiological SFAs and Glc levels - a finding referred to as 'metabolic memory'.This study examined chronic and oscillating SFAs and Glc on the inflammatory signalling pathway in human adipose tissue (AT) and adipocytes (Ads) and determined whether Ads are subject to "metabolic memory.". Abdominal (Abd) subcutaneous (Sc) explants and Ads were treated with chronic low glucose (L-Glc): 5.6 mM and high glucose (H-Glc): 17.5 mM, with low (0.2 mM) and high (2 mM) SFA for 48 h. Abd Sc explants and Ads were also exposed to the aforementioned treatment regimen for 12-h periods, with alternating rest periods of 12 h in L-Glc.Chronic treatment with L-Glc and high SFAs, H-Glc and high SFAs up-regulated key factors of the nuclear factor-κB (NFκB) pathway in Abd Sc AT and Ads (TLR4, NFκB; P

Original languageEnglish
Pages (from-to)39-50
Number of pages12
JournalJournal of Nutritional Biochemistry
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 2012

Keywords

  • Glucose
  • Human adipose tissue
  • Inflammation
  • Obesity
  • Saturated fatty acids
  • Toll-like receptors

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Fingerprint Dive into the research topics of 'Acute and chronic saturated fatty acid treatment as a key instigator of the TLR-mediated inflammatory response in human adipose tissue, in vitro'. Together they form a unique fingerprint.

Cite this