Acute lymphoblastic leukaemia associated antigen. IV Expression on non-leukaemic 'lymphoid' cells

Melvyn Greaves, Domenico Delia, George Janossy, Nicholas Rapson, Judith Chessells, Marilyn Woods, Grant Prentice

Research output: Contribution to journalArticle

Abstract

The cellular specificity of the common ALL (cALL) membrane (gp100) antigen and the p28, 33 (Ia-like) antigen has been further explored. Non-leukaemic cells with a variable and usually weak expression of the cALL antigen can be demonstrated in foetal haemopoietic tissue, in bone marrow of normal children, in the marrow of children with a variety of non-leukaemic haematologic and non-haematologic disorders. In normal children the cALL antigen positive cells appear to be restricted to the bone marrow. These cells are regularly detected in the marrow of many leukaemic (ALL, AML) and non-leukaemic children with post-chemotherapy associated lymphocytosis, in marrow transplant recipients, and in neonatal 'leukaemoid' reactions. When the number of cALL+ cells is monitored in children following cessation of maintenance chemotherapy for ALL, some prognostic correlation can be observed; in general, however, the numbers of 'lymphoid' cells bearing these leukaemia associated antigens have no clear predictive value with respect to relapse. Analysis by simultaneous markers coupled with flow microfluorimetry and sorting indicates that the majority of cALL antigen+ cells (> 90%) are positive for the p28, 33 (Ia-like) antigen but do not express markers of mature lymphocytes (T antigen, E sheep, E mouse rosettes, surface immunoglobulin), binding sites for complement and IgG or a myeloid membrane antigen. More than 90% of pre-B cells (i.e. cyt IgM+, SmIg-) are p28, 33 positive but only a small proportion (5-15%) are cALL antigen positive. These and additional data support the view that the cALL and p28, 33 (Ia) antigens are normal 'early' differentiation antigens of the lymphoid lineage.

Original languageEnglish
JournalLeukemia Research
Volume4
Issue number1
DOIs
Publication statusPublished - 1980

Fingerprint

Precursor Cell Lymphoblastic Leukemia-Lymphoma
Lymphocytes
Antigens
Bone Marrow
Histocompatibility Antigens Class II
Maintenance Chemotherapy
Lymphocytosis
B-Cell Antigen Receptors
B-Lymphoid Precursor Cells
Membranes
Viral Tumor Antigens
Differentiation Antigens
Immunoglobulin M
Sheep
Flow Cytometry
Leukemia
Fetus
Immunoglobulin G
Binding Sites
Recurrence

Keywords

  • Differentiation antigens
  • fluorescence activated cell sorter
  • haemopoietic regeneration
  • leukaemic lymphoblasts

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Greaves, M., Delia, D., Janossy, G., Rapson, N., Chessells, J., Woods, M., & Prentice, G. (1980). Acute lymphoblastic leukaemia associated antigen. IV Expression on non-leukaemic 'lymphoid' cells. Leukemia Research, 4(1). https://doi.org/10.1016/0145-2126(80)90044-2

Acute lymphoblastic leukaemia associated antigen. IV Expression on non-leukaemic 'lymphoid' cells. / Greaves, Melvyn; Delia, Domenico; Janossy, George; Rapson, Nicholas; Chessells, Judith; Woods, Marilyn; Prentice, Grant.

In: Leukemia Research, Vol. 4, No. 1, 1980.

Research output: Contribution to journalArticle

Greaves, M, Delia, D, Janossy, G, Rapson, N, Chessells, J, Woods, M & Prentice, G 1980, 'Acute lymphoblastic leukaemia associated antigen. IV Expression on non-leukaemic 'lymphoid' cells', Leukemia Research, vol. 4, no. 1. https://doi.org/10.1016/0145-2126(80)90044-2
Greaves, Melvyn ; Delia, Domenico ; Janossy, George ; Rapson, Nicholas ; Chessells, Judith ; Woods, Marilyn ; Prentice, Grant. / Acute lymphoblastic leukaemia associated antigen. IV Expression on non-leukaemic 'lymphoid' cells. In: Leukemia Research. 1980 ; Vol. 4, No. 1.
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abstract = "The cellular specificity of the common ALL (cALL) membrane (gp100) antigen and the p28, 33 (Ia-like) antigen has been further explored. Non-leukaemic cells with a variable and usually weak expression of the cALL antigen can be demonstrated in foetal haemopoietic tissue, in bone marrow of normal children, in the marrow of children with a variety of non-leukaemic haematologic and non-haematologic disorders. In normal children the cALL antigen positive cells appear to be restricted to the bone marrow. These cells are regularly detected in the marrow of many leukaemic (ALL, AML) and non-leukaemic children with post-chemotherapy associated lymphocytosis, in marrow transplant recipients, and in neonatal 'leukaemoid' reactions. When the number of cALL+ cells is monitored in children following cessation of maintenance chemotherapy for ALL, some prognostic correlation can be observed; in general, however, the numbers of 'lymphoid' cells bearing these leukaemia associated antigens have no clear predictive value with respect to relapse. Analysis by simultaneous markers coupled with flow microfluorimetry and sorting indicates that the majority of cALL antigen+ cells (> 90{\%}) are positive for the p28, 33 (Ia-like) antigen but do not express markers of mature lymphocytes (T antigen, E sheep, E mouse rosettes, surface immunoglobulin), binding sites for complement and IgG or a myeloid membrane antigen. More than 90{\%} of pre-B cells (i.e. cyt IgM+, SmIg-) are p28, 33 positive but only a small proportion (5-15{\%}) are cALL antigen positive. These and additional data support the view that the cALL and p28, 33 (Ia) antigens are normal 'early' differentiation antigens of the lymphoid lineage.",
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AB - The cellular specificity of the common ALL (cALL) membrane (gp100) antigen and the p28, 33 (Ia-like) antigen has been further explored. Non-leukaemic cells with a variable and usually weak expression of the cALL antigen can be demonstrated in foetal haemopoietic tissue, in bone marrow of normal children, in the marrow of children with a variety of non-leukaemic haematologic and non-haematologic disorders. In normal children the cALL antigen positive cells appear to be restricted to the bone marrow. These cells are regularly detected in the marrow of many leukaemic (ALL, AML) and non-leukaemic children with post-chemotherapy associated lymphocytosis, in marrow transplant recipients, and in neonatal 'leukaemoid' reactions. When the number of cALL+ cells is monitored in children following cessation of maintenance chemotherapy for ALL, some prognostic correlation can be observed; in general, however, the numbers of 'lymphoid' cells bearing these leukaemia associated antigens have no clear predictive value with respect to relapse. Analysis by simultaneous markers coupled with flow microfluorimetry and sorting indicates that the majority of cALL antigen+ cells (> 90%) are positive for the p28, 33 (Ia-like) antigen but do not express markers of mature lymphocytes (T antigen, E sheep, E mouse rosettes, surface immunoglobulin), binding sites for complement and IgG or a myeloid membrane antigen. More than 90% of pre-B cells (i.e. cyt IgM+, SmIg-) are p28, 33 positive but only a small proportion (5-15%) are cALL antigen positive. These and additional data support the view that the cALL and p28, 33 (Ia) antigens are normal 'early' differentiation antigens of the lymphoid lineage.

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