Abstract
The cellular specificity of the common ALL (cALL) membrane (gp100) antigen and the p28, 33 (Ia-like) antigen has been further explored. Non-leukaemic cells with a variable and usually weak expression of the cALL antigen can be demonstrated in foetal haemopoietic tissue, in bone marrow of normal children, in the marrow of children with a variety of non-leukaemic haematologic and non-haematologic disorders. In normal children the cALL antigen positive cells appear to be restricted to the bone marrow. These cells are regularly detected in the marrow of many leukaemic (ALL, AML) and non-leukaemic children with post-chemotherapy associated lymphocytosis, in marrow transplant recipients, and in neonatal 'leukaemoid' reactions. When the number of cALL+ cells is monitored in children following cessation of maintenance chemotherapy for ALL, some prognostic correlation can be observed; in general, however, the numbers of 'lymphoid' cells bearing these leukaemia associated antigens have no clear predictive value with respect to relapse. Analysis by simultaneous markers coupled with flow microfluorimetry and sorting indicates that the majority of cALL antigen+ cells (> 90%) are positive for the p28, 33 (Ia-like) antigen but do not express markers of mature lymphocytes (T antigen, E sheep, E mouse rosettes, surface immunoglobulin), binding sites for complement and IgG or a myeloid membrane antigen. More than 90% of pre-B cells (i.e. cyt IgM+, SmIg-) are p28, 33 positive but only a small proportion (5-15%) are cALL antigen positive. These and additional data support the view that the cALL and p28, 33 (Ia) antigens are normal 'early' differentiation antigens of the lymphoid lineage.
| Original language | English |
|---|---|
| Journal | Leukemia Research |
| Volume | 4 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1980 |
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Keywords
- Differentiation antigens
- fluorescence activated cell sorter
- haemopoietic regeneration
- leukaemic lymphoblasts
ASJC Scopus subject areas
- Cancer Research
- Hematology
- Oncology
Cite this
Acute lymphoblastic leukaemia associated antigen. IV Expression on non-leukaemic 'lymphoid' cells. / Greaves, Melvyn; Delia, Domenico; Janossy, George; Rapson, Nicholas; Chessells, Judith; Woods, Marilyn; Prentice, Grant.
In: Leukemia Research, Vol. 4, No. 1, 1980.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Acute lymphoblastic leukaemia associated antigen. IV Expression on non-leukaemic 'lymphoid' cells
AU - Greaves, Melvyn
AU - Delia, Domenico
AU - Janossy, George
AU - Rapson, Nicholas
AU - Chessells, Judith
AU - Woods, Marilyn
AU - Prentice, Grant
PY - 1980
Y1 - 1980
N2 - The cellular specificity of the common ALL (cALL) membrane (gp100) antigen and the p28, 33 (Ia-like) antigen has been further explored. Non-leukaemic cells with a variable and usually weak expression of the cALL antigen can be demonstrated in foetal haemopoietic tissue, in bone marrow of normal children, in the marrow of children with a variety of non-leukaemic haematologic and non-haematologic disorders. In normal children the cALL antigen positive cells appear to be restricted to the bone marrow. These cells are regularly detected in the marrow of many leukaemic (ALL, AML) and non-leukaemic children with post-chemotherapy associated lymphocytosis, in marrow transplant recipients, and in neonatal 'leukaemoid' reactions. When the number of cALL+ cells is monitored in children following cessation of maintenance chemotherapy for ALL, some prognostic correlation can be observed; in general, however, the numbers of 'lymphoid' cells bearing these leukaemia associated antigens have no clear predictive value with respect to relapse. Analysis by simultaneous markers coupled with flow microfluorimetry and sorting indicates that the majority of cALL antigen+ cells (> 90%) are positive for the p28, 33 (Ia-like) antigen but do not express markers of mature lymphocytes (T antigen, E sheep, E mouse rosettes, surface immunoglobulin), binding sites for complement and IgG or a myeloid membrane antigen. More than 90% of pre-B cells (i.e. cyt IgM+, SmIg-) are p28, 33 positive but only a small proportion (5-15%) are cALL antigen positive. These and additional data support the view that the cALL and p28, 33 (Ia) antigens are normal 'early' differentiation antigens of the lymphoid lineage.
AB - The cellular specificity of the common ALL (cALL) membrane (gp100) antigen and the p28, 33 (Ia-like) antigen has been further explored. Non-leukaemic cells with a variable and usually weak expression of the cALL antigen can be demonstrated in foetal haemopoietic tissue, in bone marrow of normal children, in the marrow of children with a variety of non-leukaemic haematologic and non-haematologic disorders. In normal children the cALL antigen positive cells appear to be restricted to the bone marrow. These cells are regularly detected in the marrow of many leukaemic (ALL, AML) and non-leukaemic children with post-chemotherapy associated lymphocytosis, in marrow transplant recipients, and in neonatal 'leukaemoid' reactions. When the number of cALL+ cells is monitored in children following cessation of maintenance chemotherapy for ALL, some prognostic correlation can be observed; in general, however, the numbers of 'lymphoid' cells bearing these leukaemia associated antigens have no clear predictive value with respect to relapse. Analysis by simultaneous markers coupled with flow microfluorimetry and sorting indicates that the majority of cALL antigen+ cells (> 90%) are positive for the p28, 33 (Ia-like) antigen but do not express markers of mature lymphocytes (T antigen, E sheep, E mouse rosettes, surface immunoglobulin), binding sites for complement and IgG or a myeloid membrane antigen. More than 90% of pre-B cells (i.e. cyt IgM+, SmIg-) are p28, 33 positive but only a small proportion (5-15%) are cALL antigen positive. These and additional data support the view that the cALL and p28, 33 (Ia) antigens are normal 'early' differentiation antigens of the lymphoid lineage.
KW - Differentiation antigens
KW - fluorescence activated cell sorter
KW - haemopoietic regeneration
KW - leukaemic lymphoblasts
UR - http://www.scopus.com/inward/record.url?scp=0018823110&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018823110&partnerID=8YFLogxK
U2 - 10.1016/0145-2126(80)90044-2
DO - 10.1016/0145-2126(80)90044-2
M3 - Article
C2 - 6931953
AN - SCOPUS:0018823110
VL - 4
JO - Leukemia Research
JF - Leukemia Research
SN - 0145-2126
IS - 1
ER -