Acute vitamin D3 supplementation in severe obesity: Evaluation of multimeric adiponectin

Stefania Mai, Gillian E. Walker, Roberta Vietti, Stefania Cattaldo, Chiara Mele, Lorenzo Priano, Alessandro Mauro, Gianni Bona, Gianluca Aimaretti, Massimo Scacchi, Paolo Marzullo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Obesity predisposes to vitamin D deficiency (VDD) and glucose abnormalities. It is currently debated if vitamin D administration may improve glucose homeostasis by interacting with modulators of insulin sensitivity, such as adiponectin and its oligomers. In a 4-week inpatient study on a metabolic rehabilitation program, consisting of individualized caloric restriction and aerobic physical exercise in obese subjects with VDD, we assessed the acute effects of 600,000 IU cholecalciferol given per os VD group, 12 subjects, body mass index (BMI) 42.7 ± 1.3 kg/m2) or placebo per os (PL group, 12 subjects, BMI 39.8 ± 0.9 kg/m2) on high (HWM-A), medium (MMW-A), and low molecular weight adiponectin (LMW-A), as quantified by western immunoblot (WIB) and ELISA. During the 4-week study, dieting promoted a similar magnitude of weight loss in VD and PL groups. Compared to the PL group, cholecalciferol administration increased 25(OH)Vit D levels (p < 0.001) and promoted a significant increase of HMW-A expression analyzed by WIB (p = 0.02). In parallel, a significant decrease of leptin/HMW-A ratio (p < 0.05), a biomarker of metabolic homeostasis, was observed. During the study, changes of MMW-A and LMW-A occurred independently of cholecalciferol administration, and were likely explained by weight loss. At odds with these findings, the ELISA assessment of adiponectin oligomers showed no modifications in the VD group or PL group. Current findings suggest that acute cholecalciferol administration selectively modifies HMW-A and the leptin/HMW-A ratio.

Original languageEnglish
Article number459
JournalNutrients
Volume9
Issue number5
DOIs
Publication statusPublished - May 1 2017

Fingerprint

adiponectin
Morbid Obesity
cholecalciferol
Cholecalciferol
Adiponectin
obesity
vitamin D deficiency
Vitamin D Deficiency
Leptin
leptin
body mass index
Weight Loss
homeostasis
Body Mass Index
Homeostasis
weight loss
Molecular Weight
Western Blotting
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay

Keywords

  • Multimeric adiponectin
  • Obesity
  • Vitamin D
  • Weight loss

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Acute vitamin D3 supplementation in severe obesity : Evaluation of multimeric adiponectin. / Mai, Stefania; Walker, Gillian E.; Vietti, Roberta; Cattaldo, Stefania; Mele, Chiara; Priano, Lorenzo; Mauro, Alessandro; Bona, Gianni; Aimaretti, Gianluca; Scacchi, Massimo; Marzullo, Paolo.

In: Nutrients, Vol. 9, No. 5, 459, 01.05.2017.

Research output: Contribution to journalArticle

Mai, Stefania ; Walker, Gillian E. ; Vietti, Roberta ; Cattaldo, Stefania ; Mele, Chiara ; Priano, Lorenzo ; Mauro, Alessandro ; Bona, Gianni ; Aimaretti, Gianluca ; Scacchi, Massimo ; Marzullo, Paolo. / Acute vitamin D3 supplementation in severe obesity : Evaluation of multimeric adiponectin. In: Nutrients. 2017 ; Vol. 9, No. 5.
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