AD6(8-monochloro-3-beta-diethylaminoethyl-4-methyl-7-ethoxy-carbonyl-methoxy-coumarin) reportedly exerts specific coronary vasodilatory activity and reduces platelet aggregation in laboratory animals. We investigated whether AD6 stimulates prostacyclin (PGI2) synthesis by vascular cells. AD6 treatment of rats induced a dose dependent enhancement of prostacyclin (PGI2) synthesis by rings of thoracic aorta measured by bioassay and confirmed by thin layer radio-chromatography of the products of 14C arachidonic acid(14CAA) metabolism. In vitro incubation of the drug with cultured human endothelial cells caused a concentration dependent stimulation of PGI2 production measured by radioimmunoassay of 6keto PGF1α. It is proposed that the previously described vasodilatory activity of the drug might be mediated through the stimulation of PGI2 release.
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