ADAMTS13 activity, high VWF and FVIII levels in the pathogenesis of deep vein thrombosis

Maria Teresa Pagliari, Marco Boscarino, Andrea Cairo, Ilaria Mancini, Ida Martinelli, Paolo Bucciarelli, Federica Rossi, Frits Richard Rosendaal, Flora Peyvandi

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Deep vein thrombosis (DVT) is a common multi-factorial disease with a partially understood aetiology. Although the roles of high factor (F)VIII and von Willebrand factor (VWF) levels are recognized, that of ADAMTS13 is still unclear. Aim: To assess the association between ADAMTS13 activity levels, VWF antigen (VWF:Ag) and FVIII coagulant activity (FVIII:C) levels and DVT. Materials and methods: 365 Italian DVT patients and 292 age- and sex-matched controls were considered. Plasma ADAMTS13 activity was measured using FRETS-VWF73 assay. VWF:Ag and FVIII:C were measured using immunoassay and one-stage clotting assay (ACL TOP analyzer), respectively. Quartile analyses were performed to evaluate the individual association between ADAMTS13 activity, VWF:Ag, FVIII:C and DVT. The combined effect of high VWF levels (> 4th quartile) and low ADAMTS13 levels (< 1st quartile) was evaluated using binary variables. All models were age- and sex-adjusted. Estimated risks were reported as Odds ratio (OR) with 95% confidence intervals (CI). Results: ADAMTS13 activity was lower in DVT patients (94% vs. 98% of controls). Patients with an ADAMTS13 activity <1st quartile (86%) showed a 1.6-fold increased risk of DVT (95%CI, 1.05–2.55). The combination of low ADAMTS13 activity and high VWF:Ag levels was associated with a 15–fold increased risk (95%CI, 7.80–33.80). VWF:Ag and FVIII:C were associated to DVT with a dose-response relationship. Conclusions: ADAMTS13 activity < 86% was associated with a moderate risk of DVT. The co-presence of low ADAMTS13 activity and high VWF levels resulted in a strong synergistic effect on DVT risk. The association of VWF:Ag and FVIII:C with DVT was confirmed.

Original languageEnglish
Pages (from-to)132-137
Number of pages6
JournalThrombosis Research
Volume197
DOIs
Publication statusPublished - Jan 2021

Keywords

  • ADAMTS13 human protein
  • Deep vein thrombosis
  • Factor VIII
  • Risk factor
  • von Willebrand factor

ASJC Scopus subject areas

  • Hematology

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