Adaptive changes of duodenal iron transport proteins in celiac disease

Donatella Barisani, Antonina Parafioriti, Maria Teresa Bardella, Heinz Zoller, Dario Conte, Elisabetta Armiraglio, Cristina Trovato, Robert O. Koch, Günter Weiss

Research output: Contribution to journalArticle

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Abstract

Iron deficiency is a manifestation of celiac disease (CD) usually attributed to a decreased absorptive surface, although no data on the regulation of iron transport under these conditions are currently available. Our aim was to evaluate divalent metal transporter 1 (DMT1), duodenal cytochrome b (Dcytb), ferroportin 1 (FP1), hephaestin, and transferrin receptor 1 (TfR1) expression, as well as iron regulatory protein (IRP) activity in duodenal biopsies from control, anemic, and CD patients. We studied 10 subjects with dyspepsia, 6 with iron-deficiency anemia, and 25 with CD. mRNA levels were determined by real-time PCR, protein expression by Western blotting or immunohistochemistry, and IRP activity by gel shift assay. Our results showed that DMT1, FP1, hephaestin, and TfR1 mRNA levels were significantly increased in CD patients with reduced body iron stores compared with controls, similar to what was observed in anemic patients. Protein expression paralleled the mRNAs changes. DMT1 protein expression was localized in differentiated enterocytes at the villi tips in controls, whereas with iron deficiency it was observed throughout the villi. FP1 expression was localized on the basolateral membrane of enterocytes and increased with low iron stores. TfR1 was localized in the crypts in controls but also in the villi with iron deficiency. These changes were paralleled by IRP activity, which increased in all iron-deficient subjects. We conclude that duodenal DMT1, FP1, hephaestin, and TfR1 expression and IRP activity, thus the iron absorption capacity, are upregulated in CD patients as a consequence of iron deficiency, whereas the increased enterocyte proliferation observed in CD has no effect on iron uptake regulation.

Original languageEnglish
Pages (from-to)316-325
Number of pages10
JournalPhysiol Genomics
Volume17
DOIs
Publication statusPublished - Jul 2004

Fingerprint

Celiac Disease
Carrier Proteins
Iron
Transferrin Receptors
Iron-Regulatory Proteins
Enterocytes
Messenger RNA
Iron Regulatory Protein 1
Proteins
Iron-Deficiency Anemias
Dyspepsia
Real-Time Polymerase Chain Reaction
Western Blotting
Gels
Immunohistochemistry
Biopsy
Membranes
solute carrier family 11- (proton-coupled divalent metal ion transporters), member 2
metal transporting protein 1

Keywords

  • Divalent metal transporter 1
  • Ferroportin 1
  • Iron absorption
  • Transferrin receptor 1

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Barisani, D., Parafioriti, A., Bardella, M. T., Zoller, H., Conte, D., Armiraglio, E., ... Weiss, G. (2004). Adaptive changes of duodenal iron transport proteins in celiac disease. Physiol Genomics, 17, 316-325. https://doi.org/10.1152/physiolgenomics.00211.2003

Adaptive changes of duodenal iron transport proteins in celiac disease. / Barisani, Donatella; Parafioriti, Antonina; Bardella, Maria Teresa; Zoller, Heinz; Conte, Dario; Armiraglio, Elisabetta; Trovato, Cristina; Koch, Robert O.; Weiss, Günter.

In: Physiol Genomics, Vol. 17, 07.2004, p. 316-325.

Research output: Contribution to journalArticle

Barisani, D, Parafioriti, A, Bardella, MT, Zoller, H, Conte, D, Armiraglio, E, Trovato, C, Koch, RO & Weiss, G 2004, 'Adaptive changes of duodenal iron transport proteins in celiac disease', Physiol Genomics, vol. 17, pp. 316-325. https://doi.org/10.1152/physiolgenomics.00211.2003
Barisani D, Parafioriti A, Bardella MT, Zoller H, Conte D, Armiraglio E et al. Adaptive changes of duodenal iron transport proteins in celiac disease. Physiol Genomics. 2004 Jul;17:316-325. https://doi.org/10.1152/physiolgenomics.00211.2003
Barisani, Donatella ; Parafioriti, Antonina ; Bardella, Maria Teresa ; Zoller, Heinz ; Conte, Dario ; Armiraglio, Elisabetta ; Trovato, Cristina ; Koch, Robert O. ; Weiss, Günter. / Adaptive changes of duodenal iron transport proteins in celiac disease. In: Physiol Genomics. 2004 ; Vol. 17. pp. 316-325.
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