TY - JOUR
T1 - ADAR2 editing enzyme is a novel human immunodeficiency virus-1 proviral factor
AU - Doria, Margherita
AU - Tomaselli, Sara
AU - Neri, Francesca
AU - Ciafrè, Silvia Anna
AU - Farace, Maria Giulia
AU - Michienzi, Alessandro
AU - Gallo, Angela
PY - 2011/5
Y1 - 2011/5
N2 - The adenosine deaminases acting on RNA (ADAR) enzymes catalyse conversion of adenosine to inosine in dsRNA. A positive effect of ADAR1 on human immunodeficiency virus type 1 (HIV-1) replication has recently been reported. Here, we show that another ADAR enzyme, ADAR2, positively affects the replication process of HIV-1. We found that, analogously to ADAR1, ADAR2 enhances the release of progeny virions by an editing-dependent mechanism. However, differently from the ADAR1 enzyme, ADAR2 does not increase the infectious potential of the virus. Importantly, downregulation of ADAR2 in Jurkat cells significantly impairs viral replication. Therefore, ADAR2 shares some but not all proviral functions of ADAR1. These results suggest a novel role of ADAR2 as a viral regulator.
AB - The adenosine deaminases acting on RNA (ADAR) enzymes catalyse conversion of adenosine to inosine in dsRNA. A positive effect of ADAR1 on human immunodeficiency virus type 1 (HIV-1) replication has recently been reported. Here, we show that another ADAR enzyme, ADAR2, positively affects the replication process of HIV-1. We found that, analogously to ADAR1, ADAR2 enhances the release of progeny virions by an editing-dependent mechanism. However, differently from the ADAR1 enzyme, ADAR2 does not increase the infectious potential of the virus. Importantly, downregulation of ADAR2 in Jurkat cells significantly impairs viral replication. Therefore, ADAR2 shares some but not all proviral functions of ADAR1. These results suggest a novel role of ADAR2 as a viral regulator.
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U2 - 10.1099/vir.0.028043-0
DO - 10.1099/vir.0.028043-0
M3 - Article
C2 - 21289159
AN - SCOPUS:79955620214
VL - 92
SP - 1228
EP - 1232
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 5
ER -