Additional evidence that pityriasis rosea is associated with reactivation of human herpesvirus-6 and -7

Francesco Broccolo, Francesco Drago, Anna M. Careddu, Chiara Foglieni, Laura Turbino, Clementina E. Cocuzza, Carlo Gelmetti, Paolo Lusso, Alfredo E. Rebora, Mauro S. Malnati

Research output: Contribution to journalArticlepeer-review

Abstract

To elucidate the role of human herpesvirus (HHV)-6 and -7 (HHV-7) in pityriasis rosea (PR), we measured their DNA load in plasma, peripheral blood mononuclear cells (PBMC), and tissues using a calibrated quantitative real-time PCR assay. We also studied HHV-6- and HHV-7-specific antigens in skin by immunohistochemistry and anti-HHV-7 neutralizing activity using a syncytia-inhibition test. Plasma and PBMC were obtained from 31 PR patients (14 children, 17 adults), 12 patients with other dermatites, and 36 blood donors. Skin biopsies were obtained from 15 adults with PR and 12 with other dermatites. HHV-6 and HHV-7 DNA were detected in 17% and in 39% of PR plasmas, respectively, but in no controls. HHV-7 viremia was associated with a higher PBMC load and, in adults, with systemic symptoms. HHV-7, but not HHV-6, levels in PBMC were higher in PR patients than in controls. HHV-6 and HHV-7 antigens were found only in PR skin (17% and 67% of patients analyzed, respectively), indicating a productive infection. Syncytia-neutralizing antibodies were found in PR patients and controls, but their titers were lower in patients with HHV-7 viremia. These data confirm the causal association between PR and active HHV-7 or, to a lesser extent, HHV-6 infection.

Original languageEnglish
Pages (from-to)1234-1240
Number of pages7
JournalJournal of Investigative Dermatology
Volume124
Issue number6
DOIs
Publication statusPublished - Jun 2005

Keywords

  • Herpesviruses
  • immunohistochemistry
  • pathogenesis
  • pityriasis rosea
  • Real-time PCR analysis

ASJC Scopus subject areas

  • Dermatology

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