Adenine and pyridine nucleotides in erythroid cell development

P. Ninfali, L. Baronciani, F. Sani

Research output: Contribution to journalArticlepeer-review

Abstract

Marrow-bound erythroblasts and circulating reticulocytes were considered in this study as two progressive stages of erythroid cell development. The contents of ATP, ADP, AMP, ITP, inosine, hypoxanthine, xanthine, NAD and NADP were determined in the two cell populations immediately after the extraction of the cells from the animal body and after incubations in different media. In the former case, the content of ATP, ITP and NAD was found to be markedly decreased in reticulocytes with respect to erythroblasts. In contrast, the decay of the other compounds was negligible taking into account the change of volume between the two cell populations. In the latter case, we found that erythroblasts incubated in the absence of glucose showed a marked decrease of ATP, ITP and NAD content within the first hour, while NADP was constant. In reticulocytes incubated under the same conditions only ATP decreased. In the presence of glucose, erythroblasts showed a significant decrease of ATP and ITP, while reticulocytes showed a constant content of all nucleotides. A moderate but significant decay of ATP content was also shown in erythroblasts incubated in a complete culture medium. In all cases, the lost ATP and ITP were almost completely transformed into hypoxanthine. The results indicate that (a) the content of adenine and pyridine nucleotides is significantly reduced in the transition from marrow-bound erythroblasts to circulating reticulocytes; (b) ATP content is linked to ITP in erythroblasts but not in reticulocytes and (c) erythroblasts are not able to maintain in vitro the same nucleotide content found in vivo, while reticulocytes maintain the energetic charge constant only in the presence of glucose.

Original languageEnglish
Pages (from-to)96-106
Number of pages11
JournalCellular Physiology and Biochemistry
Volume5
Issue number2
Publication statusPublished - 1995

Keywords

  • Adenine nucleotides
  • Erythroblasts
  • Purine catabolism
  • Pyridine nucleotides
  • Reticulocytes

ASJC Scopus subject areas

  • Cell Biology
  • Physiology

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