Adenosine and ADP prevent apoptosis in cultured rat cerebellar granule cells

O. V. Vitolo, M. T. Ciotti, C. Galli, T. Borsello, P. Calissano

Research output: Contribution to journalArticlepeer-review

Abstract

Cerebellar granule cells (CGCs) explanted in vitro undergo death via apoptosis when the concentration of potassium is shifted from 25 mM to 5 mM. We report that adenosine and ADP, which act as neurotransmitters and neuromodulators in the brain, exert in cultured cerebellar granule cells a specific and marked antiapoptotic action with half-maximal effect in the 10- 100 μM range. The action of adenosine is partly inhibited by the A1AR antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) and is mimicked by the A1AR agonist 2-chloro-N6-cyclopentyladenosine (CCPA), while ADP effect, that is completely blocked by the P2x, P2y receptors noncompetitive antagonist suramine, is restored in the presence of the selective P2x purinoceptors agonist β,γ-methylene-L-ATP. These findings demonstrate that adenosine and ADP markedly inhibit the program of cell death in cerebellar granule cells and suggest that such an action is mediated via interaction with, respectively, A1 and P2x receptors.

Original languageEnglish
Pages (from-to)297-301
Number of pages5
JournalBrain Research
Volume809
Issue number2
DOIs
Publication statusPublished - Nov 2 1998

Keywords

  • Adenosine
  • ADP
  • Apoptosis
  • Cerebellar granule cell
  • Purinoceptor

ASJC Scopus subject areas

  • Neuroscience(all)

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