Adenosine triphosphate acts as a paracrine signaling molecule to reduce the motility of T cells

Chiuhui Mary Wang, Cristina Ploia, Fabio Anselmi, Adelaida Sarukhan, Antonella Viola

Research output: Contribution to journalArticlepeer-review

Abstract

Organization of immune responses requires exchange of information between cells. This is achieved through either direct cell-cell contacts and establishment of temporary synapses or the release of soluble factors, such as cytokines and chemokines. Here we show a novel form of cell-to-cell communication based on adenosine triphosphate (ATP). ATP released by stimulated T cells induces P2X4/P2X7-mediated calcium waves in the neighboring lymphocytes. Our data obtained in lymph node slices suggest that, during T-cell priming, ATP acts as a paracrine messenger to reduce the motility of lymphocytes and that this may be relevant to allow optimal tissue scanning by T cells. Synopsis In reactive lymph nodes, ATP released by stimulated T cells is a paracrine signal to alarm the neighboring lymphocytes and reduce their motility. ATP released by stimulated T cells induces calcium waves in non-adhering, bystander T cells. P2X4 and P2X7 receptors are both involved in paracrine ATP signaling among lymphocytes. ATP signaling inhibits bystander cell motility during T-cell activation in lymph nodes. ATP released by activated T cells induces P2X4/P2X7-mediated calcium waves in neighboring lymphocytes leading to reduced lymphocytes motility that promotes more effective antigen scanning.

Original languageEnglish
Pages (from-to)1354-1364
Number of pages11
JournalEMBO Journal
Volume33
Issue number12
DOIs
Publication statusPublished - Jun 17 2014

Keywords

  • adenosine triphosphate
  • calcium wave
  • cell migration
  • imaging
  • T cells

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

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