Adenovirus-mediated gene transfer of wild-type p53 results in melanoma cell apoptosis in intro and in vivo

C. Cirielli, T. Riccioni, C. Yang, R. Pili, T. Gloe, J. Chang, K. Inyaku, A. Passaniti, M. C. Capogrossi

Research output: Contribution to journalArticlepeer-review


Gene transfer techniques may provide efficient treatment for a variety of malignant neoplasms. A replication-deficient adenovirus (Ad) vector which carries the cDNA for wild-type p53 (AdCMV.p53) was tested for its in vitro and in vivo effects on the growth of murine melanoma cell line B16-G3.26 and human melanoma cell line SK-MEL-24. The growth of B16-G3.26 cells infected with AdCMV.p53 was inhibited when compared to the uninfected cells or cells infected with the control vector AdCMV.NLSβgal. Similarly, the growth of SK-MEL-24 cells infected with AdCMV.p53 was also below that of AdCMV.NLβgal-infected and uninfected controls. DNA laddering using agarose gel electrophoresis and in situ labeling of DNA fragmentation (TUNEL) showed that AdCMV.p53 infected murine and human melanoma cells underwent apoptosis. Nude mice injected s.c. either with B16-G3.26 cells or with SK-MEL-24 cells developed localized tumors. These tumors were subsequently infiltrated with either AdCMV.p53, AdCMV.NLSβgal or saline alone. One week after infection, B16-G3.26 tumors exposed to AdCMV.p53 were 2.5 times smaller than control tumors and exhibited DNA fragmentation. A similar growth-inhibitory effect of AdCMV.p53 was observed with SK-MEL-24 tumors. Thus, Ad-mediated wild-type p53 overexpression resulted in melanoma cell apoptosis and inhibition of melanoma growth in vitro and in vivo. These gene therapy approaches may be useful in targeting rapidly growing, malignant melanomas in a clinical setting.

Original languageEnglish
Pages (from-to)673-679
Number of pages7
JournalInternational Journal of Cancer
Issue number5
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


Dive into the research topics of 'Adenovirus-mediated gene transfer of wild-type p53 results in melanoma cell apoptosis in intro and in vivo'. Together they form a unique fingerprint.

Cite this