Adenovirus-mediated wild-type p53 expression induces apoptosis and suppresses tumorigenesis of prostatic tumor cells

C. Yang, C. Cirielli, M. C. Capogrossi, A. Passaniti

Research output: Contribution to journalArticle

Abstract

The use of replication-deficient adenoviral vectors in gene therapy may become a powerful method to achieve efficient but safe transfer of antitumor agents. Introduction of the wild-type p55 gene into tumor cells has, in general, been associated with growth suppression. In this study, infection of androgen-independent human prostate Tsu-pr1 cells lacking functional p53 alleles resulted in high levels of p53 protein within 10-15 h. Cells infected with AdCMV.p53 detached from the substratum, condensed, and exhibited fragmentation of nuclear DNA into nucleosomal units consistent with the process of apoptosis. These effects were evident within 24 h after infection, and the majority of cells had undergone apoptosis by 48 h, whereas cells infected with AdCMV.NLSβGal continued to proliferate. Uninfected or AdCMV.NLSβGal-infected Tsu-pr1 cells formed tumors in nude mice within 3 weeks after implantation, whereas AdCMV.p53-infected cells failed to form tumors during this period. Therefore, adenoviral-mediated antitumor therapy using the p53 gene is an efficient method to inhibit prostate tumor growth, and agents that target the cellular programmed cell death pathway may be useful in clinical applications.

Original languageEnglish
Pages (from-to)4210-4213
Number of pages4
JournalCancer Research
Volume55
Issue number19
Publication statusPublished - 1995

    Fingerprint

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Yang, C., Cirielli, C., Capogrossi, M. C., & Passaniti, A. (1995). Adenovirus-mediated wild-type p53 expression induces apoptosis and suppresses tumorigenesis of prostatic tumor cells. Cancer Research, 55(19), 4210-4213.