Adhesion molecule expression on B-cell chronic lymphocytic leukemia cells: Malignant cell phenotypes define distinct disease subsets

Giulio De Rossi, Daniela Zarcone, Francesca Mauro, Giannamaria Cerruti, Claudya Tenca, Antonio Puccetti, Franco Mandelli, Carlo E. Grossi

Research output: Contribution to journalArticle

Abstract

Expression of surface adhesion molecules of the Ig super-family (CD54 and CD58), of the integrin family (β1, β2, and β3 chains), of the selectin family (L-selectin), and of the lymphocyte homing receptor (CD44) was analyzed on B-cell chronic lymphocytic leukemia (B-CLL) cells from 74 patients. The aim of the study was the definition of phenotypically distinct disease subsets and the correlation of adhesion molecule phenotypes with clinical parameters. Expression of CD58 on B-CLL cells defined more advanced disease stages. In comparison with β chain-positive cases, patients whose cells did not express β1, β2, and β3 integrin chains fell into the most favorable prognostic group, with lower lymphocytosis and the absence of splenomegaly, diffuse bone marrow infiltration, and therapy requirement. A novel finding was the expression of β3 chains on cells from a minority (12 of 74) of B-CLL cases. β3 chains were always coexpressed with β1, and β2 chains. Two-color immunofluorescence analyses of adhesion molecules such as α×β2 integrin (LeuM5) and L-selectin (Leu8) showed that these markers were detectable on variable proportions of leukemic cells, thus confirming the intraclonal phenotypic heterogeneity of B-CLL. Differences in the intensity of CD44 expression were also shown among the various B-CLL clones. Finally, no major variations were shown by comparison of adhesion molecule phenotypes of leukemic cells simultaneously obtained from blood and bone marrow, and of CD5+ versus CD5- clones.

Original languageEnglish
Pages (from-to)2679-2687
Number of pages9
JournalBlood
Volume81
Issue number10
Publication statusPublished - May 15 1993

ASJC Scopus subject areas

  • Hematology

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    De Rossi, G., Zarcone, D., Mauro, F., Cerruti, G., Tenca, C., Puccetti, A., Mandelli, F., & Grossi, C. E. (1993). Adhesion molecule expression on B-cell chronic lymphocytic leukemia cells: Malignant cell phenotypes define distinct disease subsets. Blood, 81(10), 2679-2687.