Adhesion of a Staphylococcus aureus strain to biomaterials does not select methicillin-resistant mutants

L. Montanaro, D. Cavedagna, L. Baldassarri, C. R. Arciola

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Bacterial adhesion to polymethylmethacrylate and to silicon elastomer, materials frequently used in clinical applications, has been investigated to assess whether adhesion selects methicillin-resistant mutants in the bacterial population in contact with the materials. The methicillin susceptibility of a susceptible Staphylococcus aureus (ATCC 25923) was measured by a modification of plate antibiogram Kirby-Bauer method, which allows optimised detection of small variations in antibiotic susceptibility. In both adherent and non-adherent bacterial subpopulations, the presence of mecA gene, which encodes for the protein PBP 2a responsible for methicillin resistance was searched for by Polymerase Chain Reaction (PCR). The contact with the two polymers did not induce in the bacteria population any phenotypic increase in methicillin resistance, or the selection of mutants carrying the mecA gene.

Original languageEnglish
Pages (from-to)57-61
Number of pages5
JournalNew Microbiologica
Volume24
Issue number1
Publication statusPublished - Jan 2001

Fingerprint

Methicillin Resistance
Biocompatible Materials
Staphylococcus aureus
Elastomers
Bacterial Adhesion
Methicillin
Microbial Sensitivity Tests
Polymethyl Methacrylate
Silicon
Population
Genes
Polymers
Anti-Bacterial Agents
Bacteria
Polymerase Chain Reaction
Proteins

Keywords

  • Bacterial adhesion
  • mecA gene
  • Methicillin resistance
  • Polymethylmethacrylate
  • Silicon elastomer
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Adhesion of a Staphylococcus aureus strain to biomaterials does not select methicillin-resistant mutants. / Montanaro, L.; Cavedagna, D.; Baldassarri, L.; Arciola, C. R.

In: New Microbiologica, Vol. 24, No. 1, 01.2001, p. 57-61.

Research output: Contribution to journalArticle

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