Adhesive protein expression on endothelial cells after contact in vitro with polyethylene terephthalate coated with pyrolytic carbon

E. Cenni, D. Granchi, C. R. Arciola, G. Ciapetti, L. Savarino, S. Stea, D. Cavedagna, A. Di Leo, A. Pizzoferrato

Research output: Contribution to journalArticlepeer-review

Abstract

This research aims at evaluating the expression of some adhesive proteins on endothelial cell surface after contact with polyethylene terephthalate coated with pyrolytic carbon (PET + PC). Twenty-two different cultures of human umbilical vein endothelial cells (HUVECs) were put in contact with PET + PC. Both HUVECs grown without the biomaterial and HUVECs incubated with endotoxin were used as control. After 24 h, platelet endothelial cell adhesion molecule-1 (PECAM-1), endothelial leucocyte adhesion molecule-1 (ELAM-1), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) were evaluated on the cells by monoclonal antibodies and flow cytometry. In agreement with the literature, after 24 h the culture incubated with endotoxin determined a significant increase in the percentage of positive cells for ELAM-1, a significant increase in fluorescence intensity for ICAM-1, and a significant increase in the percentage of positive cells and fluorescence intensity for VCAM-1. After 24 h of culture with PET + PC, no significant variations in the antigens examined were observed. This demonstrates that such material does not activate in vitro the proteins involved in the adhesion between leucocytes and endothelium or in the adhesion between endothelial cells themselves.

Original languageEnglish
Pages (from-to)1223-1227
Number of pages5
JournalBiomaterials
Volume16
Issue number16
DOIs
Publication statusPublished - 1995

Keywords

  • adhesins
  • Endothelium
  • flow cytometry
  • pyrolytic carbon

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Mechanics of Materials
  • Biomaterials
  • Ceramics and Composites
  • Biotechnology
  • Biomedical Engineering

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