Adipose mesenchymal stem cells protect chondrocytes from degeneration associated with osteoarthritis

Marie Maumus, Cristina Manferdini, Karine Toupet, Julie Anne Peyrafitte, Rosanna Ferreira, Andrea Facchini, Elena Gabusi, Philippe Bourin, Christian Jorgensen, Gina Lisignoli, Danièle Noël

Research output: Contribution to journalArticle

Abstract

Our work aimed at evaluating the role of adipose stem cells (ASC) on chondrocytes from osteoarthritic (OA) patients and identifying the mediators involved. We used primary chondrocytes, ASCs from different sources and bone marrow mesenchymal stromal cells (MSC) from OA donors. ASCs or MSCs were co-cultured with chondrocytes in a minimal medium and using cell culture inserts. Under these conditions, ASCs did not affect the proliferation of chondrocytes but significantly decreased camptothecin-induced apoptosis. Both MSCs and ASCs from different sources allowed chondrocytes in the cocultures maintaining a stable expression of markers specific for a mature phenotype, while expression of hypertrophic and fibrotic markers was decreased. A number of factors known to regulate the chondrocyte phenotype (IL-1β, IL-1RA, TNF-α) and matrix remodeling (TIMP-1 and -2, MMP-1 and -9, TSP-1) were not affected. However, a significant decrease of TGF-β1 secretion by chondrocytes and induction of HGF secretion by ASCs was observed. Addition of a neutralizing anti-HGF antibody reversed the anti-fibrotic effect of ASCs whereas hypertrophic markers were not modulated. In summary, ASCs are an interesting source of stem cells for efficiently reducing hypertrophy and dedifferentiation of chondrocytes, at least partly via the secretion of HGF. This supports the interest of using these cells in therapies for osteo-articular diseases.

Original languageEnglish
Pages (from-to)834-844
Number of pages11
JournalStem Cell Research
Volume11
Issue number2
DOIs
Publication statusPublished - Sep 2013

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Chondrocytes
Mesenchymal Stromal Cells
Osteoarthritis
Stem Cells
Thrombospondin 1
Phenotype
Tissue Inhibitor of Metalloproteinase-2
Camptothecin
Tissue Inhibitor of Metalloproteinase-1
Cell- and Tissue-Based Therapy
Coculture Techniques
Neutralizing Antibodies
Matrix Metalloproteinases
Interleukin-1
Hypertrophy
Anti-Idiotypic Antibodies
Cell Culture Techniques
Joints
Tissue Donors
Apoptosis

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)

Cite this

Adipose mesenchymal stem cells protect chondrocytes from degeneration associated with osteoarthritis. / Maumus, Marie; Manferdini, Cristina; Toupet, Karine; Peyrafitte, Julie Anne; Ferreira, Rosanna; Facchini, Andrea; Gabusi, Elena; Bourin, Philippe; Jorgensen, Christian; Lisignoli, Gina; Noël, Danièle.

In: Stem Cell Research, Vol. 11, No. 2, 09.2013, p. 834-844.

Research output: Contribution to journalArticle

Maumus, M, Manferdini, C, Toupet, K, Peyrafitte, JA, Ferreira, R, Facchini, A, Gabusi, E, Bourin, P, Jorgensen, C, Lisignoli, G & Noël, D 2013, 'Adipose mesenchymal stem cells protect chondrocytes from degeneration associated with osteoarthritis', Stem Cell Research, vol. 11, no. 2, pp. 834-844. https://doi.org/10.1016/j.scr.2013.05.008
Maumus, Marie ; Manferdini, Cristina ; Toupet, Karine ; Peyrafitte, Julie Anne ; Ferreira, Rosanna ; Facchini, Andrea ; Gabusi, Elena ; Bourin, Philippe ; Jorgensen, Christian ; Lisignoli, Gina ; Noël, Danièle. / Adipose mesenchymal stem cells protect chondrocytes from degeneration associated with osteoarthritis. In: Stem Cell Research. 2013 ; Vol. 11, No. 2. pp. 834-844.
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