TY - JOUR
T1 - Adipose stromal/stem cells assist fat transplantation reducing necrosis and increasing graft performance
AU - Piccinno, Maria Serena
AU - Veronesi, Elena
AU - Loschi, Pietro
AU - Pignatti, Marco
AU - Murgia, Alba
AU - Grisendi, Giulia
AU - Castelli, Ilaria
AU - Bernabei, Daniela
AU - Candini, Olivia
AU - Conte, Pierfranco
AU - Paolucci, Paolo
AU - Horwitz, Edwin M.
AU - De Santis, Giorgio
AU - Iughetti, Lorenzo
AU - Dominici, Massimo
PY - 2013
Y1 - 2013
N2 - Autologous fat transfer (AFT) is a procedure for adipose tissue (AT) repair after trauma, burns, post-tumor resections and lipodystrophies still negatively impacted by the lack of graft persistence. The reasons behind this poor outcome are unclear and seem to involve damages in either harvested/transplanted mature adipocytes or on their mesenchymal progenitors, namely adipose stromal/stem cells (ASC), and due to post-transplant AT apoptosis and involution. A rabbit subcutaneous AT regeneration model was here developed to first evaluate graft quality at different times after implant focusing on related parameters, such as necrosis and vasculogenesis. Standard AFT was compared with a strategy where purified autologous ASC, combined with hyaluronic acid (HA), assisted AFT. Five million of autologous ex vivo isolated CD29+, CD90+, CD49e+ ASC, loaded into HA, enriched 1 ml of AT generating an early significant protective effect in reducing AFT necrosis and increasing vasculogenesis with a preservation of transplanted AT architecture. This beneficial impact of ASC assisted AFT was then confirmed at three months with a robust lipopreservation and no signs of cellular transformation. By a novel ASC assisted AFT approach we ensure a reduction in early cell death favoring an enduring graft performance possibly for a more stable benefit in patients.
AB - Autologous fat transfer (AFT) is a procedure for adipose tissue (AT) repair after trauma, burns, post-tumor resections and lipodystrophies still negatively impacted by the lack of graft persistence. The reasons behind this poor outcome are unclear and seem to involve damages in either harvested/transplanted mature adipocytes or on their mesenchymal progenitors, namely adipose stromal/stem cells (ASC), and due to post-transplant AT apoptosis and involution. A rabbit subcutaneous AT regeneration model was here developed to first evaluate graft quality at different times after implant focusing on related parameters, such as necrosis and vasculogenesis. Standard AFT was compared with a strategy where purified autologous ASC, combined with hyaluronic acid (HA), assisted AFT. Five million of autologous ex vivo isolated CD29+, CD90+, CD49e+ ASC, loaded into HA, enriched 1 ml of AT generating an early significant protective effect in reducing AFT necrosis and increasing vasculogenesis with a preservation of transplanted AT architecture. This beneficial impact of ASC assisted AFT was then confirmed at three months with a robust lipopreservation and no signs of cellular transformation. By a novel ASC assisted AFT approach we ensure a reduction in early cell death favoring an enduring graft performance possibly for a more stable benefit in patients.
KW - Adipose tissue
KW - Autologous fat transfer
KW - Lipopreservation
KW - Mesenchymal stromal/stem cells
KW - Necrosis
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U2 - 10.1007/s10495-013-0878-7
DO - 10.1007/s10495-013-0878-7
M3 - Article
C2 - 23828239
AN - SCOPUS:84885426515
VL - 18
SP - 1274
EP - 1289
JO - Apoptosis : an international journal on programmed cell death
JF - Apoptosis : an international journal on programmed cell death
SN - 1360-8185
IS - 10
ER -