Administration of PLP 139-151 primes T cells distinct from those spontaneously responsive in vitro to this antigen

Romina Penitente, Chiara Nicolò, Peter Van Den Elzen, Gabriele Di Sante, Chiara Agrati, Francesca Aloisi, Eli E. Sercarz, Francesco Ria

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Abstract

We examined the TCR repertoire used by naive SJL mice in their in vitro spontaneous response to proteolipid protein (PLP) 139-151 by Vβ-Jβ spectratyping and compared it to that used after immunization with the peptide. T cells from immunized mice use the public rearrangement Vβ 10-Jβ 1.1, but naive mice do not; in contrast, TCR CDR3-β rearrangements of Vβ 18-Jβ 1.2 and Vβ 9-Jβ l.2 consistently are associated with the spontaneous response. T cells involved in spontaneous and induced responses can each recognize PLP I39-I51 presented in vivo, but its s.c. administration has different consequences for the two repertoires. Four days after immunization, T cells associated with spontaneous responsiveness appear in the draining lymph nodes but disappear by day 10 and never appear elsewhere. Simultaneously, Vβ 10-Jβ 1.1 T cells are likewise activated in the lymph nodes by day 4 and spread to the spleen by day 10. Eight- to 10-wk-old naive mice use a narrower repertoire of TCRs than do immunized age-matched mice. Induced Vβ 10-Jβ 1.1 T cells home to the CNS during experimental autoimmune encephalomyelitis, whereas we failed to detect Vβ 18-Jβ 1.2 and Vβ 19-Jβ 1.2 TCR rearrangements in the CNS. Thus, we observe that administration of PLP 139-151 primes a T cell repertoire distinct from the one responsible for spontaneous responsiveness. This "immunized" repertoire substitutes for the naive one and becomes dominant at the time of disease onset.

Original languageEnglish
Pages (from-to)6611-6622
Number of pages12
JournalJournal of Immunology
Volume180
Issue number10
Publication statusPublished - 2008

ASJC Scopus subject areas

  • Immunology

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