We examined the TCR repertoire used by naive SJL mice in their in vitro spontaneous response to proteolipid protein (PLP) 139-151 by Vβ-Jβ spectratyping and compared it to that used after immunization with the peptide. T cells from immunized mice use the public rearrangement Vβ 10-Jβ 1.1, but naive mice do not; in contrast, TCR CDR3-β rearrangements of Vβ 18-Jβ 1.2 and Vβ 9-Jβ l.2 consistently are associated with the spontaneous response. T cells involved in spontaneous and induced responses can each recognize PLP I39-I51 presented in vivo, but its s.c. administration has different consequences for the two repertoires. Four days after immunization, T cells associated with spontaneous responsiveness appear in the draining lymph nodes but disappear by day 10 and never appear elsewhere. Simultaneously, Vβ 10-Jβ 1.1 T cells are likewise activated in the lymph nodes by day 4 and spread to the spleen by day 10. Eight- to 10-wk-old naive mice use a narrower repertoire of TCRs than do immunized age-matched mice. Induced Vβ 10-Jβ 1.1 T cells home to the CNS during experimental autoimmune encephalomyelitis, whereas we failed to detect Vβ 18-Jβ 1.2 and Vβ 19-Jβ 1.2 TCR rearrangements in the CNS. Thus, we observe that administration of PLP 139-151 primes a T cell repertoire distinct from the one responsible for spontaneous responsiveness. This "immunized" repertoire substitutes for the naive one and becomes dominant at the time of disease onset.
|Number of pages||12|
|Journal||Journal of Immunology|
|Publication status||Published - 2008|
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