Administration of the antitumor drug mitoguazone protects normal thymocytes against spontaneous and etoposide-induced apoptosis

M. E. Ferioli, M. G. Bottone, C. Soldani, C. Pellicciari

Research output: Contribution to journalArticle

Abstract

The suggestion has been made that polyamines may be involved in the control of cell death, since exceedingly high or low levels induce apoptosis in different cell systems. For a deeper insight into the relationship between apoptosis and polyamine metabolism, we investigated in vitro the effect on rat thymocytes of mitoguazone (MGBG, which inhibits S-adenosylmethionine decarboxylase, i.e. a key enzyme in the polyamine biosynthetic pathway). Thymocytes were selected as an especially suitable model system, since they undergo spontaneous apoptosis in vivo and can be easily induced to apoptose in vitro by etoposide, used here as an apoptogenic agent. MGBG protected thymocytes from both spontaneous and drug-induced apoptosis, and this protective effect was associated with a decrease in polyamine oxidase activity and total polyamine levels.

Original languageEnglish
Pages (from-to)2767-2773
Number of pages7
JournalCellular and Molecular Life Sciences
Volume61
Issue number21
DOIs
Publication statusPublished - Nov 2004

Keywords

  • Apoptosis
  • Etoposide
  • Mitoguazone
  • Polyamine
  • Polyamine oxidase
  • Thymocyte

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

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