Adolescent feline heart contains a population of small, proliferative ventricular myocytes with immature physiological properties

Xiongwen Chen, Rachel M. Wilson, Hajime Kubo, Remus M. Berretta, David M. Harris, Xiaoying Zhang, Naser Jaleel, Scott M. MacDonnell, Claudia Bearzi, Jochen Tillmanns, Irina Trofimova, Toru Hosoda, Federico Mosna, Leanne Cribbs, Annarosa Leri, Jan Kajstura, Piero Anversa, Steven R. Houser

Research output: Contribution to journalArticle

Abstract

Recent studies suggest that rather than being terminally differentiated, the adult heart is a self-renewing organ with the capacity to generate new myocytes from cardiac stem/progenitor cells (CS/PCs). This study examined the hypotheses that new myocytes are generated during adolescent growth, to increase myocyte number, and these newly formed myocytes are initially small, mononucleated, proliferation competent, and have immature properties. Ventricular myocytes (VMs) and cKit (stem cell receptor) CS/PCs were isolated from 11- and 22-week feline hearts. Bromodeoxyuridine incorporation (in vivo) and p16 immunostaining were measured to assess myocyte cell cycle activity and senescence, respectively. Telomerase activity, contractions, Ca transients, and electrophysiology were compared in small mononucleated (SMMs) and large binucleated (LBMs) myocytes. Heart mass increased by 101% during adolescent growth, but left ventricular myocyte volume only increased by 77%. Most VMs were binucleated (87% versus 12% mononucleated) and larger than mononucleated myocytes. A greater percentage of SMMs was bromodeoxyuridine positive (SMMs versus LBMs: 3.1% versus 0.8%; P

Original languageEnglish
Pages (from-to)536-544
Number of pages9
JournalCirculation Research
Volume100
Issue number4
DOIs
Publication statusPublished - Mar 2007

Keywords

  • Calcium
  • Cardiac stem cells
  • New ventricular myocytes
  • T-type calcium current

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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    Chen, X., Wilson, R. M., Kubo, H., Berretta, R. M., Harris, D. M., Zhang, X., Jaleel, N., MacDonnell, S. M., Bearzi, C., Tillmanns, J., Trofimova, I., Hosoda, T., Mosna, F., Cribbs, L., Leri, A., Kajstura, J., Anversa, P., & Houser, S. R. (2007). Adolescent feline heart contains a population of small, proliferative ventricular myocytes with immature physiological properties. Circulation Research, 100(4), 536-544. https://doi.org/10.1161/01.RES.0000259560.39234.99