Adoptive immunotherapy with antigen-specific T cells during extracorporeal membrane oxygenation (ECMO) for adenovirus-related respiratory failure in a child given haploidentical stem cell transplantation

Matteo Di Nardo, Giuseppina Li Pira, Antonio Amodeo, Corrado Cecchetti, Ezio Giorda, Stefano Ceccarelli, Letizia P. Brescia, Nicola Pirozzi, Sergio Rutella, Franco Locatelli, Alice Bertaina

Research output: Contribution to journalArticlepeer-review

Abstract

We report on the successful infusion of human adenovirus (HAdV)-specific T cells in a child with congenital amegakaryocytic thrombocytopenia, given T-cell-depleted hematopoietic stem cell transplantation (HSCT) from the HLA-haploidentical mother during extracorporeal membrane oxygenation (ECMO) for severe HAdV-related respiratory failure. Donor-derived, interferon (IFN)-γ-secreting HAdV-specific T cells were enriched using the cytokine capture assay, after in vitro stimulation with overlapping peptides from the immunodominant HAdV5 hexon protein. Two weeks after T-cell transfer, viral load decreased and ECMO was discontinued. T-cell responses to HAdV antigens were documented after four weeks and were associated with viral clearance, immune reconstitution and clinical amelioration.

Original languageEnglish
Pages (from-to)376-379
Number of pages4
JournalPediatric Blood and Cancer
Volume61
Issue number2
DOIs
Publication statusPublished - Feb 2014

Keywords

  • Adenovirus
  • ECMO
  • Immune recovery
  • Immunotherapy
  • Stem cell transplantation
  • T lymphocyte

ASJC Scopus subject areas

  • Oncology
  • Pediatrics, Perinatology, and Child Health
  • Hematology

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