Adoptive immunotherapy with tumor-infiltrating lymphocytes and subcutaneous recombinant interleukin-2 plus interferon alfa-2a for melanoma patients with nonresectable distant disease: A phase I/II pilot trial

Paola Queirolo, Marco Ponte, Marco Gipponi, Ferdinando Cafiero, Alberto Peressini, Claudia Semino, Gabriella Pietra, Rita Lionetto, Stefania Vecchio, Iole Ribizzi, Giovanni Melioli, Mario R. Sertoli

Research output: Contribution to journalArticle

Abstract

Background: On the basis of our previous experience, we designed this study to determine the activity and toxicity of outpatient treatment with autologous tumor-infiltrating lymphocytes (TIL) together with intermediate- dose recombinant interleukin-2 (rIL-2) and low-dose recombinant interferon alfa-2a (rIFN-α2a), for patients with metastatic melanoma. Methods: Between April 1992 and October 1994, we processed 38 melanoma samples derived from 36 patients with metastases. Proliferative cultures of expanded lymphocytes (TIL) were infused only once into patients with metastatic melanoma, rIL-2 was administered subcutaneously for 1 month, starting on the day of TIL infusion, at an escalating dose of 6-18 x 106 IU/m2/day for the first week and at the maximum-tolerated dose for the subsequent 3 weeks and then, after a 15-day interval, for 1 week/month for 3 months, rIFN-α2a was administered subcutaneously at 3 x 106 IU three times each week until progression. Results: Of 38 melanoma samples, 19 (50%) resulted in proliferative cultures and were infused. The median number of expanded lymphocytes was 18 x 109 (range, 1-43 x 109), and the median period of culture was 52 days (range, 45-60). rIL-2 was administered at doses ranging between 6 and 18 x 106 IU/m2/day. Toxicity was mild or moderate, and no life-threatening side effects were encountered. Two of 19 treated patients experienced complete responses of their metastatic sites (soft tissue), 10 had stable disease, and 7 showed progressive disease. The response rate was 11% (95% confidence interval, 2-35%). Conclusions: Outpatient treatment with TIL plus rIL-2 and rIFN-α2a is feasible, although, within the context of the small sample size, the activity of the combination was no different from the reported activity of any of the components used alone.

Original languageEnglish
Pages (from-to)272-278
Number of pages7
JournalAnnals of Surgical Oncology
Volume6
Issue number3
DOIs
Publication statusPublished - Apr 1999

Keywords

  • Adoptive immunotherapy
  • Interferon alfa-2a
  • Interleukin-2
  • Tumor-infiltrating lymphocytes

ASJC Scopus subject areas

  • Surgery
  • Oncology

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