Adult acute non-lymphoblastic leukaemia

Reliability and prognostic significance of pretreatment bone marrow S-phase size determined by flow cytofluorometry

A. Riccardi, M. Danova, C. Montecucco, G. Ucci, E. Cassano, M. Giordano, G. Mazzini, P. Giordano

Research output: Contribution to journalArticle

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Abstract

46 adult patients with non-lymphoblastic acute leukaemia (AnLL) had pretreatment proliferative activity of bone marrow (BM) blasts determined simultaneously with propidium iodide DNA flow cytofluorometry (as the percentage of cells with DNA content intermediate between the diploid and the tetraploid values, 2n-4n cell %) and in vitro tritiated thymidine cytoautoradiography (as the labelling index, LI). They were then treated with the same chemotherapy regimen, including 2-3 courses of sequential vincristine, arabinosylcytosine and adriamycin, for response induction, and monthly courses of different cytostatics for maintenance. Median 2n-4n cell % was 9.9 and median LI was 5.9. A close linear relationship (r = 0.913, p <0.001) between the two parameters was found. Patients having a 2n-4n cell % greater than 15.6 responded more often to chemotherapy than patients having a lower percentage (p <0.05) but also experienced a shorter duration of first response (p <0.05). Overall survival was longer in patients with 2n-4n cell % greater than 15.6 (p <0.02). Multiple regression analysis indicates that 2n-4n cell % is a statistically significant (p <0.05) independent factor correlating with duration of response in adult AnLL.

Original languageEnglish
Pages (from-to)11-17
Number of pages7
JournalScandinavian Journal of Haematology
Volume36
Issue number1
Publication statusPublished - 1986

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S Phase
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Flow Cytometry
Bone Marrow
Drug Therapy
Tetraploidy
Propidium
DNA
Cytarabine
Cytostatic Agents
Vincristine
Diploidy
Doxorubicin
Thymidine
Leukemia
Regression Analysis
Maintenance
Survival

ASJC Scopus subject areas

  • Hematology

Cite this

Adult acute non-lymphoblastic leukaemia : Reliability and prognostic significance of pretreatment bone marrow S-phase size determined by flow cytofluorometry. / Riccardi, A.; Danova, M.; Montecucco, C.; Ucci, G.; Cassano, E.; Giordano, M.; Mazzini, G.; Giordano, P.

In: Scandinavian Journal of Haematology, Vol. 36, No. 1, 1986, p. 11-17.

Research output: Contribution to journalArticle

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abstract = "46 adult patients with non-lymphoblastic acute leukaemia (AnLL) had pretreatment proliferative activity of bone marrow (BM) blasts determined simultaneously with propidium iodide DNA flow cytofluorometry (as the percentage of cells with DNA content intermediate between the diploid and the tetraploid values, 2n-4n cell {\%}) and in vitro tritiated thymidine cytoautoradiography (as the labelling index, LI). They were then treated with the same chemotherapy regimen, including 2-3 courses of sequential vincristine, arabinosylcytosine and adriamycin, for response induction, and monthly courses of different cytostatics for maintenance. Median 2n-4n cell {\%} was 9.9 and median LI was 5.9. A close linear relationship (r = 0.913, p <0.001) between the two parameters was found. Patients having a 2n-4n cell {\%} greater than 15.6 responded more often to chemotherapy than patients having a lower percentage (p <0.05) but also experienced a shorter duration of first response (p <0.05). Overall survival was longer in patients with 2n-4n cell {\%} greater than 15.6 (p <0.02). Multiple regression analysis indicates that 2n-4n cell {\%} is a statistically significant (p <0.05) independent factor correlating with duration of response in adult AnLL.",
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T2 - Reliability and prognostic significance of pretreatment bone marrow S-phase size determined by flow cytofluorometry

AU - Riccardi, A.

AU - Danova, M.

AU - Montecucco, C.

AU - Ucci, G.

AU - Cassano, E.

AU - Giordano, M.

AU - Mazzini, G.

AU - Giordano, P.

PY - 1986

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N2 - 46 adult patients with non-lymphoblastic acute leukaemia (AnLL) had pretreatment proliferative activity of bone marrow (BM) blasts determined simultaneously with propidium iodide DNA flow cytofluorometry (as the percentage of cells with DNA content intermediate between the diploid and the tetraploid values, 2n-4n cell %) and in vitro tritiated thymidine cytoautoradiography (as the labelling index, LI). They were then treated with the same chemotherapy regimen, including 2-3 courses of sequential vincristine, arabinosylcytosine and adriamycin, for response induction, and monthly courses of different cytostatics for maintenance. Median 2n-4n cell % was 9.9 and median LI was 5.9. A close linear relationship (r = 0.913, p <0.001) between the two parameters was found. Patients having a 2n-4n cell % greater than 15.6 responded more often to chemotherapy than patients having a lower percentage (p <0.05) but also experienced a shorter duration of first response (p <0.05). Overall survival was longer in patients with 2n-4n cell % greater than 15.6 (p <0.02). Multiple regression analysis indicates that 2n-4n cell % is a statistically significant (p <0.05) independent factor correlating with duration of response in adult AnLL.

AB - 46 adult patients with non-lymphoblastic acute leukaemia (AnLL) had pretreatment proliferative activity of bone marrow (BM) blasts determined simultaneously with propidium iodide DNA flow cytofluorometry (as the percentage of cells with DNA content intermediate between the diploid and the tetraploid values, 2n-4n cell %) and in vitro tritiated thymidine cytoautoradiography (as the labelling index, LI). They were then treated with the same chemotherapy regimen, including 2-3 courses of sequential vincristine, arabinosylcytosine and adriamycin, for response induction, and monthly courses of different cytostatics for maintenance. Median 2n-4n cell % was 9.9 and median LI was 5.9. A close linear relationship (r = 0.913, p <0.001) between the two parameters was found. Patients having a 2n-4n cell % greater than 15.6 responded more often to chemotherapy than patients having a lower percentage (p <0.05) but also experienced a shorter duration of first response (p <0.05). Overall survival was longer in patients with 2n-4n cell % greater than 15.6 (p <0.02). Multiple regression analysis indicates that 2n-4n cell % is a statistically significant (p <0.05) independent factor correlating with duration of response in adult AnLL.

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