Adult murine CNS stem cells express aquaporin channels

Caterina A M La Porta, Patrizia Gena, Angela Gritti, Umberto Fascio, Maria Svelto, Giuseppe Calamita

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background information. Fluid homoeostasis is of critical importance in many functions of the CNS (central nervous system) as indicated by the fact that dysregulation of cell volume underlies clinical conditions such as brain oedema and hypoxia. Water balance is also important during neurogenesis as neural stem cells move considerable amounts of water into or out of the cell to rapidly change their volume during differentiation. Consistent with the relevance of water transport in CNS, multiple AQP (aquaporin) water channels have been recognized and partially characterized in brain cell function. However, the presence and distribution of AQPs in CNS stem cells has not yet been assessed. Results. In the present study, we investigate the expression and subcellular localization of AQPs in murine ANSCs (adult neural stem cells). Considerable AQP8 mRNAs were found in ANSCs where, as expected, the transcript of two additional AQPs, AQP4 and AQP9, was also detected. Immunoblotting with subcellular membrane fractions of ANSCs showed predominant expression of AQP8 in the mitochondria-enriched fraction. This result was consistent with the spotted immunoreactivity profile encountered within the ANSCs by confocal immunofluorescence. Conclusions. AQP8 may have a role in mitochondrial volume regulation during ANSC differentiation. Recognition of AQPs in ANSCs Is a step forward in our knowledge of water homoeostasis in the CNS and provides useful information for the purposes of stem cell technology.

Original languageEnglish
Pages (from-to)89-94
Number of pages6
JournalBiology of the cell / under the auspices of the European Cell Biology Organization
Volume98
Issue number2
DOIs
Publication statusPublished - Feb 2006

Fingerprint

Aquaporins
Neural Stem Cells
Adult Stem Cells
Stem Cells
Central Nervous System
Water
Homeostasis
Mitochondrial Size
Brain Hypoxia
Subcellular Fractions
Neurogenesis
Brain Edema
Cell Size
Immunoblotting
Fluorescent Antibody Technique
Cell Differentiation
Mitochondria
Technology
Messenger RNA
Membranes

Keywords

  • AQP8
  • Mitochondria
  • Neurogenesis
  • Oedema
  • Water transport

ASJC Scopus subject areas

  • Cell Biology

Cite this

Adult murine CNS stem cells express aquaporin channels. / La Porta, Caterina A M; Gena, Patrizia; Gritti, Angela; Fascio, Umberto; Svelto, Maria; Calamita, Giuseppe.

In: Biology of the cell / under the auspices of the European Cell Biology Organization, Vol. 98, No. 2, 02.2006, p. 89-94.

Research output: Contribution to journalArticle

La Porta, Caterina A M ; Gena, Patrizia ; Gritti, Angela ; Fascio, Umberto ; Svelto, Maria ; Calamita, Giuseppe. / Adult murine CNS stem cells express aquaporin channels. In: Biology of the cell / under the auspices of the European Cell Biology Organization. 2006 ; Vol. 98, No. 2. pp. 89-94.
@article{50732bfecfd546b6aa2344ebd4cb8265,
title = "Adult murine CNS stem cells express aquaporin channels",
abstract = "Background information. Fluid homoeostasis is of critical importance in many functions of the CNS (central nervous system) as indicated by the fact that dysregulation of cell volume underlies clinical conditions such as brain oedema and hypoxia. Water balance is also important during neurogenesis as neural stem cells move considerable amounts of water into or out of the cell to rapidly change their volume during differentiation. Consistent with the relevance of water transport in CNS, multiple AQP (aquaporin) water channels have been recognized and partially characterized in brain cell function. However, the presence and distribution of AQPs in CNS stem cells has not yet been assessed. Results. In the present study, we investigate the expression and subcellular localization of AQPs in murine ANSCs (adult neural stem cells). Considerable AQP8 mRNAs were found in ANSCs where, as expected, the transcript of two additional AQPs, AQP4 and AQP9, was also detected. Immunoblotting with subcellular membrane fractions of ANSCs showed predominant expression of AQP8 in the mitochondria-enriched fraction. This result was consistent with the spotted immunoreactivity profile encountered within the ANSCs by confocal immunofluorescence. Conclusions. AQP8 may have a role in mitochondrial volume regulation during ANSC differentiation. Recognition of AQPs in ANSCs Is a step forward in our knowledge of water homoeostasis in the CNS and provides useful information for the purposes of stem cell technology.",
keywords = "AQP8, Mitochondria, Neurogenesis, Oedema, Water transport",
author = "{La Porta}, {Caterina A M} and Patrizia Gena and Angela Gritti and Umberto Fascio and Maria Svelto and Giuseppe Calamita",
year = "2006",
month = "2",
doi = "10.1042/BC20040153",
language = "English",
volume = "98",
pages = "89--94",
journal = "Biology of the Cell",
issn = "0248-4900",
publisher = "Portland Press Ltd.",
number = "2",

}

TY - JOUR

T1 - Adult murine CNS stem cells express aquaporin channels

AU - La Porta, Caterina A M

AU - Gena, Patrizia

AU - Gritti, Angela

AU - Fascio, Umberto

AU - Svelto, Maria

AU - Calamita, Giuseppe

PY - 2006/2

Y1 - 2006/2

N2 - Background information. Fluid homoeostasis is of critical importance in many functions of the CNS (central nervous system) as indicated by the fact that dysregulation of cell volume underlies clinical conditions such as brain oedema and hypoxia. Water balance is also important during neurogenesis as neural stem cells move considerable amounts of water into or out of the cell to rapidly change their volume during differentiation. Consistent with the relevance of water transport in CNS, multiple AQP (aquaporin) water channels have been recognized and partially characterized in brain cell function. However, the presence and distribution of AQPs in CNS stem cells has not yet been assessed. Results. In the present study, we investigate the expression and subcellular localization of AQPs in murine ANSCs (adult neural stem cells). Considerable AQP8 mRNAs were found in ANSCs where, as expected, the transcript of two additional AQPs, AQP4 and AQP9, was also detected. Immunoblotting with subcellular membrane fractions of ANSCs showed predominant expression of AQP8 in the mitochondria-enriched fraction. This result was consistent with the spotted immunoreactivity profile encountered within the ANSCs by confocal immunofluorescence. Conclusions. AQP8 may have a role in mitochondrial volume regulation during ANSC differentiation. Recognition of AQPs in ANSCs Is a step forward in our knowledge of water homoeostasis in the CNS and provides useful information for the purposes of stem cell technology.

AB - Background information. Fluid homoeostasis is of critical importance in many functions of the CNS (central nervous system) as indicated by the fact that dysregulation of cell volume underlies clinical conditions such as brain oedema and hypoxia. Water balance is also important during neurogenesis as neural stem cells move considerable amounts of water into or out of the cell to rapidly change their volume during differentiation. Consistent with the relevance of water transport in CNS, multiple AQP (aquaporin) water channels have been recognized and partially characterized in brain cell function. However, the presence and distribution of AQPs in CNS stem cells has not yet been assessed. Results. In the present study, we investigate the expression and subcellular localization of AQPs in murine ANSCs (adult neural stem cells). Considerable AQP8 mRNAs were found in ANSCs where, as expected, the transcript of two additional AQPs, AQP4 and AQP9, was also detected. Immunoblotting with subcellular membrane fractions of ANSCs showed predominant expression of AQP8 in the mitochondria-enriched fraction. This result was consistent with the spotted immunoreactivity profile encountered within the ANSCs by confocal immunofluorescence. Conclusions. AQP8 may have a role in mitochondrial volume regulation during ANSC differentiation. Recognition of AQPs in ANSCs Is a step forward in our knowledge of water homoeostasis in the CNS and provides useful information for the purposes of stem cell technology.

KW - AQP8

KW - Mitochondria

KW - Neurogenesis

KW - Oedema

KW - Water transport

UR - http://www.scopus.com/inward/record.url?scp=32544453847&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=32544453847&partnerID=8YFLogxK

U2 - 10.1042/BC20040153

DO - 10.1042/BC20040153

M3 - Article

C2 - 15907198

AN - SCOPUS:32544453847

VL - 98

SP - 89

EP - 94

JO - Biology of the Cell

JF - Biology of the Cell

SN - 0248-4900

IS - 2

ER -