Advanced methods to study the cross talk between fibro-adipogenic progenitors and muscle stem cells

Luca Tucciarone, Usue Etxaniz, Martina Sandoná, Silvia Consalvi, Pier Lorenzo Puri, Valentina Saccone

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Functional interactions between muscle (satellite) stem cells—MuSCs—and other cellular components of their niche (the fibro-adipogenic progenitors—FAPs) coordinate regeneration of injured as well as diseased skeletal muscles. These interactions are largely mediated by secretory networks, whose integrity is critical to determine whether repair occurs by compensatory regeneration leading to formation of new contractile fibers, or by maladaptive formation of fibrotic scars and fat infiltration. Here we provide the description of methods for isolation of FAPs and MuSCs from muscles of wild type and dystrophic mice, and protocols of cocultures as well as MuSC’s exposure to FAP- derived exosomes. These methods and protocols can be exploited in murine models of acute muscle injury to investigate salient features of physiological repair, and in models of muscular diseases to identify dysregulated networks that compromise functional interactions between cellular components of the regeneration environment during disease progression. We predict that exporting these procedures to patient-derived muscle samples will contribute to advance our understanding of human skeletal myogenesis and related disorders.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages231-256
Number of pages26
Volume1687
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1687
ISSN (Print)1064-3745

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Keywords

  • Exosomes
  • Fibro-adipogenic progenitors
  • Satellite cells
  • Skeletal muscle
  • Transwell cocultures

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Tucciarone, L., Etxaniz, U., Sandoná, M., Consalvi, S., Puri, P. L., & Saccone, V. (2018). Advanced methods to study the cross talk between fibro-adipogenic progenitors and muscle stem cells. In Methods in Molecular Biology (Vol. 1687, pp. 231-256). (Methods in Molecular Biology; Vol. 1687). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7374-3_17