Advanced microscopy analysis of the micro-nanoscale architecture of human menisci

V Vetri, K Dragnevski, M Tkaczyk, M Zingales, Gregorio Marchiori, N F Lopomo, Stefano Zaffagnini, Alice Bondi, J A Kennedy, D W Murray, O Barrera

Research output: Contribution to journalArticle

Abstract

The complex inhomogeneous architecture of the human meniscal tissue at the micro and nano scale in the absence of artefacts introduced by sample treatments has not yet been fully revealed. The knowledge of the internal structure organization is essential to understand the mechanical functionality of the meniscus and its relationship with the tissue's complex structure. In this work, we investigated human meniscal tissue structure using up-to-date non-invasive imaging techniques, based on multiphoton fluorescence and quantitative second harmonic generation microscopy complemented with Environmental Scanning Electron Microscopy measurements. Observations on 50 meniscal samples extracted from 6 human menisci (3 lateral and 3 medial) revealed fundamental features of structural morphology and allowed us to quantitatively describe the 3D organisation of elastin and collagen fibres bundles. 3D regular waves of collagen bundles are arranged in "honeycomb-like" cells that are comprised of pores surrounded by the collagen and elastin network at the micro-scale. This type of arrangement propagates from macro to the nanoscale.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 10 2019

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Microscopy
Collagen
Elastin
Tibial Meniscus
Electron Scanning Microscopy
Artifacts
Fluorescence
Meniscus

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Vetri, V., Dragnevski, K., Tkaczyk, M., Zingales, M., Marchiori, G., Lopomo, N. F., ... Barrera, O. (2019). Advanced microscopy analysis of the micro-nanoscale architecture of human menisci. Scientific Reports, 9(1), 1-13. https://doi.org/10.1038/s41598-019-55243-2

Advanced microscopy analysis of the micro-nanoscale architecture of human menisci. / Vetri, V; Dragnevski, K; Tkaczyk, M; Zingales, M; Marchiori, Gregorio; Lopomo, N F; Zaffagnini, Stefano; Bondi, Alice; Kennedy, J A; Murray, D W; Barrera, O.

In: Scientific Reports, Vol. 9, No. 1, 10.12.2019, p. 1-13.

Research output: Contribution to journalArticle

Vetri, V, Dragnevski, K, Tkaczyk, M, Zingales, M, Marchiori, G, Lopomo, NF, Zaffagnini, S, Bondi, A, Kennedy, JA, Murray, DW & Barrera, O 2019, 'Advanced microscopy analysis of the micro-nanoscale architecture of human menisci', Scientific Reports, vol. 9, no. 1, pp. 1-13. https://doi.org/10.1038/s41598-019-55243-2
Vetri V, Dragnevski K, Tkaczyk M, Zingales M, Marchiori G, Lopomo NF et al. Advanced microscopy analysis of the micro-nanoscale architecture of human menisci. Scientific Reports. 2019 Dec 10;9(1):1-13. https://doi.org/10.1038/s41598-019-55243-2
Vetri, V ; Dragnevski, K ; Tkaczyk, M ; Zingales, M ; Marchiori, Gregorio ; Lopomo, N F ; Zaffagnini, Stefano ; Bondi, Alice ; Kennedy, J A ; Murray, D W ; Barrera, O. / Advanced microscopy analysis of the micro-nanoscale architecture of human menisci. In: Scientific Reports. 2019 ; Vol. 9, No. 1. pp. 1-13.
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