Advances in proteomic techniques for biomarker discovery in COPD

Begoña Casado, Maurizio Luisetti, Paolo Iadarola

Research output: Contribution to journalArticlepeer-review


Chronic obstructive pulmonary disease (COPD) is a disorder characterized by chronic inflammation of the lung with airflow obstruction and progressive deterioration of pulmonary function. The need to discover and validate biomarkers as prognostic tools of development and progression of the disease has received further support with the advent of proteomic techniques. Liquid chromatography-mass spectrometry (LC/MS) and gel electrophoresis-mass spectrometry (2-DE/MS) have been applied to investigate the proteome of a number of lung-origin samples, including sputum, bronchoalveolar lavage fluid, exhaled-breath condensate, cells and biopsies from COPD patients. In particular, 2-DE and MS are the main proteomic approaches with 2-DE presenting the major approach for quantitative proteomics. The molecules identified as potential biomarkers of COPD may represent a preliminary step for better comprehension of the mechanisms involved in the onset/progression of the disease.

Original languageEnglish
Pages (from-to)111-123
Number of pages13
JournalExpert Review of Clinical Immunology
Issue number1
Publication statusPublished - Jan 2011


  • BALF
  • COPD
  • exhaled breath
  • induced sputum
  • mass spectrometry
  • proteomics
  • validation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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