Advances in proteomic techniques for biomarker discovery in COPD

Begoña Casado, Maurizio Luisetti, Paolo Iadarola

Research output: Contribution to journalArticle

Abstract

Chronic obstructive pulmonary disease (COPD) is a disorder characterized by chronic inflammation of the lung with airflow obstruction and progressive deterioration of pulmonary function. The need to discover and validate biomarkers as prognostic tools of development and progression of the disease has received further support with the advent of proteomic techniques. Liquid chromatography-mass spectrometry (LC/MS) and gel electrophoresis-mass spectrometry (2-DE/MS) have been applied to investigate the proteome of a number of lung-origin samples, including sputum, bronchoalveolar lavage fluid, exhaled-breath condensate, cells and biopsies from COPD patients. In particular, 2-DE and MS are the main proteomic approaches with 2-DE presenting the major approach for quantitative proteomics. The molecules identified as potential biomarkers of COPD may represent a preliminary step for better comprehension of the mechanisms involved in the onset/progression of the disease.

Original languageEnglish
Pages (from-to)111-123
Number of pages13
JournalExpert Review of Clinical Immunology
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 2011

Keywords

  • BALF
  • COPD
  • exhaled breath
  • induced sputum
  • mass spectrometry
  • proteomics
  • validation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Advances in proteomic techniques for biomarker discovery in COPD'. Together they form a unique fingerprint.

  • Cite this