Advances in the understanding of malignant transformation of keratinocytes: An immunohistochemical study

M. G. Tucci, A. Offidani, G. Lucarini, L. Simonelli, S. Amati, A. Cellini, G. Biagini, G. Ricotti

Research output: Contribution to journalArticlepeer-review


Aim: We set out to investigate the interactions between malignant transformation of keratinocytes, presence of oncoproteins and immunosurveillance in squamous cell carcinoma (SCC) and in a preneoplastic lesion, actinic keratosis (AK). Methods: Samples of SCC, AK and normal skin (NS) were subjected to quantitative analysis using the following antibodies: anti-p53, Ki67, OKT6, OK-DR, B7/BB1, anti-CD54, anti-CD11, OKT3, OKT4, OKT8; positivity for ras-p21, EGFr and bcl-2 was evaluated by semiquantitative analysis. Results: Oncoprotein alterations and increased keratinocyte proliferative activity were observed both in AK and SCC. The number of Langerhans cells (CD1a+ cells) was similar in the two lesions but lower in SCC compared to AK. The proportion of CD1a+-B7/BB1+ cells was slightly higher in AK and SCC than in NS. The Langerhans cells expressed the HLA-DR antigen in all groups. Values were highest in AK and NS, and quite low in SCC. Cytotoxic T lymphocytes were more numerous in SCC than in AK and NS. Interestingly, the total CD4/CD8 ratio was much lower in SCC than in AK and NS, which indicates an increase in the CD8+ subpopulation in samples of SCC. In the epithelia of SCC samples there were a considerable number of B7/BB1+ keratinocytes. Conclusions: We suggest that alterations in the immunodefence mechanisms have an important role in the transformation of AK into SCC, and that these changes affect not only lymphocytes, but also professional (i.e., Langerhans cells) and non-professional (i.e., keratinocytes) antigen presenting cells.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalJournal of the European Academy of Dermatology and Venereology
Issue number2
Publication statusPublished - 1998


  • Actinic keratosis
  • Immunosurveillance
  • Langerhans cells
  • Oncoproteins
  • Squamous-cell carcinoma

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases


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