Aflatoxin B1 Cytotoxicity in Neurons in Culture

Paola Bonsi, Maura Palmery, Gabriella Augusti-Tocco

Research output: Contribution to journalArticle

Abstract

Aflatoxin B1 (AFB1), a metabolite produced by Aspergillus flavus and Aspergillus parasiticus, is mainly known for its strong hepatotoxic and hepatocarcinogenic actions. Acute and reversible effects due to exposure to aflatoxin and the presence of aflatoxins in various human tissues and organs have also been reported. In particular, aflatoxin M1 (a metabolite of AFB1) has been identified in human brain tissue, and a syndrome characterised by encephalopathy has been observed in humans poisoned by AFB1. As a first approach to the study of the neurotoxicity of AFB1, we used the human neuronal cell lines, SKNMC and SKNSH. The data reported show clearly that AFB1 is capable of interacting directly with neuronal cells and causing a decrease in cell number following the addition of toxin to the culture. Decrease in cell survival is dependent on the toxin concentration, on time of exposure, and on cell density. The cytotoxic response of these cells has been compared to the effects of AFB1 on hepatoma cells and spinal cord motor neurons. Postmitotic neurons are also susceptible to AFB1 toxicity, although to a lower extent than proliferating cells. A non-proliferating state thus appears to lower, but not destroy, neuron sensitivity to the toxin.

Original languageEnglish
Pages (from-to)533-540
Number of pages8
JournalATLA Alternatives to Laboratory Animals
Volume24
Issue number4
Publication statusPublished - Jul 1996

Fingerprint

Aflatoxin B1
aflatoxin B1
Cytotoxicity
Cell culture
Neurons
cytotoxicity
neurons
Aflatoxins
toxins
Aspergillus
Metabolites
aflatoxins
cells
Cell Count
Aflatoxin M1
Cells
Tissue
metabolites
aflatoxin M1
Aspergillus flavus

Keywords

  • Aflatoxin B
  • Chick spinal cord
  • Human neuronal cell lines

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Aflatoxin B1 Cytotoxicity in Neurons in Culture. / Bonsi, Paola; Palmery, Maura; Augusti-Tocco, Gabriella.

In: ATLA Alternatives to Laboratory Animals, Vol. 24, No. 4, 07.1996, p. 533-540.

Research output: Contribution to journalArticle

Bonsi, P, Palmery, M & Augusti-Tocco, G 1996, 'Aflatoxin B1 Cytotoxicity in Neurons in Culture', ATLA Alternatives to Laboratory Animals, vol. 24, no. 4, pp. 533-540.
Bonsi, Paola ; Palmery, Maura ; Augusti-Tocco, Gabriella. / Aflatoxin B1 Cytotoxicity in Neurons in Culture. In: ATLA Alternatives to Laboratory Animals. 1996 ; Vol. 24, No. 4. pp. 533-540.
@article{8df947bf76fe46589cb986086f18426e,
title = "Aflatoxin B1 Cytotoxicity in Neurons in Culture",
abstract = "Aflatoxin B1 (AFB1), a metabolite produced by Aspergillus flavus and Aspergillus parasiticus, is mainly known for its strong hepatotoxic and hepatocarcinogenic actions. Acute and reversible effects due to exposure to aflatoxin and the presence of aflatoxins in various human tissues and organs have also been reported. In particular, aflatoxin M1 (a metabolite of AFB1) has been identified in human brain tissue, and a syndrome characterised by encephalopathy has been observed in humans poisoned by AFB1. As a first approach to the study of the neurotoxicity of AFB1, we used the human neuronal cell lines, SKNMC and SKNSH. The data reported show clearly that AFB1 is capable of interacting directly with neuronal cells and causing a decrease in cell number following the addition of toxin to the culture. Decrease in cell survival is dependent on the toxin concentration, on time of exposure, and on cell density. The cytotoxic response of these cells has been compared to the effects of AFB1 on hepatoma cells and spinal cord motor neurons. Postmitotic neurons are also susceptible to AFB1 toxicity, although to a lower extent than proliferating cells. A non-proliferating state thus appears to lower, but not destroy, neuron sensitivity to the toxin.",
keywords = "Aflatoxin B, Chick spinal cord, Human neuronal cell lines",
author = "Paola Bonsi and Maura Palmery and Gabriella Augusti-Tocco",
year = "1996",
month = "7",
language = "English",
volume = "24",
pages = "533--540",
journal = "ATLA Alternatives to Laboratory Animals",
issn = "0261-1929",
publisher = "FRAME",
number = "4",

}

TY - JOUR

T1 - Aflatoxin B1 Cytotoxicity in Neurons in Culture

AU - Bonsi, Paola

AU - Palmery, Maura

AU - Augusti-Tocco, Gabriella

PY - 1996/7

Y1 - 1996/7

N2 - Aflatoxin B1 (AFB1), a metabolite produced by Aspergillus flavus and Aspergillus parasiticus, is mainly known for its strong hepatotoxic and hepatocarcinogenic actions. Acute and reversible effects due to exposure to aflatoxin and the presence of aflatoxins in various human tissues and organs have also been reported. In particular, aflatoxin M1 (a metabolite of AFB1) has been identified in human brain tissue, and a syndrome characterised by encephalopathy has been observed in humans poisoned by AFB1. As a first approach to the study of the neurotoxicity of AFB1, we used the human neuronal cell lines, SKNMC and SKNSH. The data reported show clearly that AFB1 is capable of interacting directly with neuronal cells and causing a decrease in cell number following the addition of toxin to the culture. Decrease in cell survival is dependent on the toxin concentration, on time of exposure, and on cell density. The cytotoxic response of these cells has been compared to the effects of AFB1 on hepatoma cells and spinal cord motor neurons. Postmitotic neurons are also susceptible to AFB1 toxicity, although to a lower extent than proliferating cells. A non-proliferating state thus appears to lower, but not destroy, neuron sensitivity to the toxin.

AB - Aflatoxin B1 (AFB1), a metabolite produced by Aspergillus flavus and Aspergillus parasiticus, is mainly known for its strong hepatotoxic and hepatocarcinogenic actions. Acute and reversible effects due to exposure to aflatoxin and the presence of aflatoxins in various human tissues and organs have also been reported. In particular, aflatoxin M1 (a metabolite of AFB1) has been identified in human brain tissue, and a syndrome characterised by encephalopathy has been observed in humans poisoned by AFB1. As a first approach to the study of the neurotoxicity of AFB1, we used the human neuronal cell lines, SKNMC and SKNSH. The data reported show clearly that AFB1 is capable of interacting directly with neuronal cells and causing a decrease in cell number following the addition of toxin to the culture. Decrease in cell survival is dependent on the toxin concentration, on time of exposure, and on cell density. The cytotoxic response of these cells has been compared to the effects of AFB1 on hepatoma cells and spinal cord motor neurons. Postmitotic neurons are also susceptible to AFB1 toxicity, although to a lower extent than proliferating cells. A non-proliferating state thus appears to lower, but not destroy, neuron sensitivity to the toxin.

KW - Aflatoxin B

KW - Chick spinal cord

KW - Human neuronal cell lines

UR - http://www.scopus.com/inward/record.url?scp=0006204254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0006204254&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0006204254

VL - 24

SP - 533

EP - 540

JO - ATLA Alternatives to Laboratory Animals

JF - ATLA Alternatives to Laboratory Animals

SN - 0261-1929

IS - 4

ER -