Age- and gender-related alterations of the number and clonogenic capacity of circulating CD34+ progenitor cells

Raffaella Moresi, Silvia Tesei, Laura Costarelli, Claudio Viticchi, Rosalia Stecconi, Giovanni Bernardini, Mauro Provinciali

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The aim of this study was to evaluate the peripheral representation and the clonogenic capacity of CD34+ progenitor cells from 130 healthy subjects (80 females and 50 males) ranging in age from 16 to 100 years. We demonstrated that the absolute number of circulating CD34+ cells progressively and significantly decreased with advancing age, with a 2-fold reduction in subjects aged more than 80 years. The number of granulocyte-macrophagic (CFU-GM), erytroid (BFU-E), and mixed (CFU-GEMM) colonies which developed from the number of CD34+ purified cells per ml, progressively and significantly decreased with advancing age. The reduction of both CD34+ cell number and clonogenic capacity during aging was statistically significant in males but not in females. When evaluated on a per cell bases, a significant age-related decrease in the number of CFU-GM colonies was observed in female but not in male subjects. Our study demonstrates the influence of gender on age-related alterations of the number and clonogenic capacity of CD34+ cells in the peripheral blood. This evidence deserves particular consideration for the future planning of stem cell therapy in age-associated debilitating diseases.

Original languageEnglish
Pages (from-to)185-192
Number of pages8
JournalBiogerontology
Volume6
Issue number3
DOIs
Publication statusPublished - May 2005

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Stem Cells
Granulocyte-Macrophage Progenitor Cells
Myeloid Progenitor Cells
Erythroid Precursor Cells
Cell- and Tissue-Based Therapy
Granulocytes
Healthy Volunteers
Cell Count

Keywords

  • Aging
  • Cytofluorimetry
  • Gender
  • Human
  • Stem cells

ASJC Scopus subject areas

  • Geriatrics and Gerontology

Cite this

Age- and gender-related alterations of the number and clonogenic capacity of circulating CD34+ progenitor cells. / Moresi, Raffaella; Tesei, Silvia; Costarelli, Laura; Viticchi, Claudio; Stecconi, Rosalia; Bernardini, Giovanni; Provinciali, Mauro.

In: Biogerontology, Vol. 6, No. 3, 05.2005, p. 185-192.

Research output: Contribution to journalArticle

Moresi, Raffaella ; Tesei, Silvia ; Costarelli, Laura ; Viticchi, Claudio ; Stecconi, Rosalia ; Bernardini, Giovanni ; Provinciali, Mauro. / Age- and gender-related alterations of the number and clonogenic capacity of circulating CD34+ progenitor cells. In: Biogerontology. 2005 ; Vol. 6, No. 3. pp. 185-192.
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