Age-dependent changes in the susceptibility to apoptosis of peripheral blood CD4+ and CD8+ T lymphocytes with virgin or memory phenotype

Stefano Salvioli, Miriam Capri, Elena Scarcella, Sandro Mangherini, Irma Faranca, Vittorio Volterra, Diana De Ronchi, Marina Marini, Massimiliano Bonafè, Claudio Franceschi, Daniela Monti

Research output: Contribution to journalArticle

Abstract

Susceptibility to apoptosis changes with age and most of the available data on lymphocytes refer to mitogen stimulated cells. We studied this susceptibility in quiescent, purified CD4+ or CD8+ T cells from a group of Italian old people compared with a group of young people. We found that an apoptotic agent such as 2-deoxy-D-ribose (dRib), which acts via glutathione depletion and oxidative stress, was more effective in CD4+ T cells from young donors, while no difference was found in CD8+ T cells. On the contrary, another agent such as TNF-α, which acts via receptor engagement, was more effective in CD8+ T cells from old subjects, and no difference was found in CD4+ T cells. When marker of activation-memory were investigated, no difference between young and old subjects was found when dRib was used. Differently, when TNF-α was used, memory and activated CD4+ T cells from old donors were less sensitive than younger counterparts, while memory CD8+ T cells from old donors were more sensitive than younger counterparts. This suggests that age-related changes in susceptibility to apoptosis of resting T cells largely depend on the type of the apoptotic stimulus which is used as well as on the memory phenotype of the cells. These results may also account, at least in part, for the deep remodelling of T cell repertoire that occurs during ageing.

Original languageEnglish
Pages (from-to)409-418
Number of pages10
JournalMechanisms of Ageing and Development
Volume124
Issue number4
DOIs
Publication statusPublished - Apr 1 2003

Keywords

  • 2-Deoxy-D-ribose
  • Aging
  • Apoptosis
  • Immunosenescence
  • Memory T lymphocytes
  • TNF-α
  • Virgin T lymphocytes

ASJC Scopus subject areas

  • Ageing
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Fingerprint Dive into the research topics of 'Age-dependent changes in the susceptibility to apoptosis of peripheral blood CD4+ and CD8+ T lymphocytes with virgin or memory phenotype'. Together they form a unique fingerprint.

  • Cite this

    Salvioli, S., Capri, M., Scarcella, E., Mangherini, S., Faranca, I., Volterra, V., De Ronchi, D., Marini, M., Bonafè, M., Franceschi, C., & Monti, D. (2003). Age-dependent changes in the susceptibility to apoptosis of peripheral blood CD4+ and CD8+ T lymphocytes with virgin or memory phenotype. Mechanisms of Ageing and Development, 124(4), 409-418. https://doi.org/10.1016/S0047-6374(03)00016-2